about
Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutansEvolution of highly active enzymes by homology-independent recombination.Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p.A method for functional mapping of protein-protein binding domain by preferential amplification of the shortest amplicon using PCR.High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV.Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification.A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis.Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter.Emulsion culture: a miniaturized library screening system based on micro-droplets in an emulsified medium.Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library.Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor.Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides.High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of "difficult-to-express" secretory recombinant proteins.Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae.Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.Regulation of RraA, a protein inhibitor of RNase E-mediated RNA decay.Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: sub-milligram production of difficult-to-express secretory protein.Stabilization of affinity-tagged recombinant protein during/after its production in a cell-free system using wheat-germ extract.Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes.Phosphatase-immunodepleted cell-free protein synthesis system.High cell-density expression system: a novel method for extracellular production of difficult-to-express proteins.Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptidesIn vitro protein biosynthesis using ribosome and foreign mRNA. An approach to construct a protein biosynthesizerA long-lived batch reaction system of cell-free protein synthesisA trimmed viral cap-independent translation enhancing sequence for rapid in vitro gene expressionImportance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticusSoy peptides enhance heterologous membrane protein productivity during the exponential growth phase of Saccharomyces cerevisiaeA method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression
P50
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P50
description
hulumtues
@sq
researcher
@en
wetenschapper
@nl
հետազոտող
@hy
name
Yasuaki Kawarasaki
@ast
Yasuaki Kawarasaki
@en
Yasuaki Kawarasaki
@es
Yasuaki Kawarasaki
@nl
Yasuaki Kawarasaki
@sl
type
label
Yasuaki Kawarasaki
@ast
Yasuaki Kawarasaki
@en
Yasuaki Kawarasaki
@es
Yasuaki Kawarasaki
@nl
Yasuaki Kawarasaki
@sl
prefLabel
Yasuaki Kawarasaki
@ast
Yasuaki Kawarasaki
@en
Yasuaki Kawarasaki
@es
Yasuaki Kawarasaki
@nl
Yasuaki Kawarasaki
@sl
P1053
J-5910-2013
P106
P1153
7003997914
P21
P31
P3829
P496
0000-0002-7143-3159
P569
2000-01-01T00:00:00Z