Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
about
Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineeringIdentification of the active site of poly(A)-specific ribonuclease by site-directed mutagenesis and Fe(2+)-mediated cleavageThe active site of the DNA repair endonuclease XPF-ERCC1 forms a highly conserved nuclease motif.Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinaseHoming endonucleases: structural and functional insight into the catalysts of intron/intein mobilityPhotocross-linking of the homing endonuclease PI-SceI to its recognition sequence.Mutational analysis of highly conserved aspartate residues essential to the catalytic core of the piggyBac transposaseDetection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking.An unspliced group I intron in 23S rRNA links Chlamydiales, chloroplasts, and mitochondria.A novel engineered meganuclease induces homologous recombination in yeast and mammalian cellsEngineering a Nickase on the Homing Endonuclease I-DmoI Scaffold.Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity.The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.Biochemical and mutagenic analysis of I-CreII reveals distinct but important roles for both the H-N-H and GIY-YIG motifsFunctional characterization of isoschizomeric His-Cys box homing endonucleases from Naegleria.Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp(122) and Asp(193) are crucial to the double-stranded DNA cleavage activity whereas Asp(218) is not.Chemical probing shows that the intron-encoded endonuclease I-SceI distorts DNA through binding in monomeric form to its homing site.Chimeras of the homing endonuclease PI-SceI and the homologous Candida tropicalis intein: a study to explore the possibility of exchanging DNA-binding modules to obtain highly specific endonucleases with altered specificity.
P2860
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P2860
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
description
1997 nî lūn-bûn
@nan
1997年の論文
@ja
1997年論文
@yue
1997年論文
@zh-hant
1997年論文
@zh-hk
1997年論文
@zh-mo
1997年論文
@zh-tw
1997年论文
@wuu
1997年论文
@zh
1997年论文
@zh-cn
name
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
@en
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
@nl
type
label
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
@en
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
@nl
prefLabel
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
@en
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.
@nl
P2860
P356
P1433
P1476
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases
@en
P2093
Garrett RA
P2860
P304
P356
10.1093/EMBOJ/16.11.3272
P407
P577
1997-06-01T00:00:00Z