Obelin from the bioluminescent marine hydroid Obelia geniculata: cloning, expression, and comparison of some properties with those of other Ca2+-regulated photoproteins.
about
Genomic organization, evolution, and expression of photoprotein and opsin genes in Mnemiopsis leidyi: a new view of ctenophore photocytesAll three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelinCrystal structures of the F88Y obelin mutant before and after bioluminescence provide molecular insight into spectral tuning among hydromedusan photoproteinsImpedance responses reveal β₂-adrenergic receptor signaling pluridimensionality and allow classification of ligands with distinct signaling profilesCloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme.Thermostable mutants of the photoprotein aequorin obtained by in vitro evolution.CcbP, a calcium-binding protein from Anabaena sp. PCC 7120, provides evidence that calcium ions regulate heterocyst differentiation.Hyperactive Arg39Lys mutated mnemiopsin: implication of positively charged residue in chromophore binding cavity.The light-sensitive photoprotein berovin from the bioluminescent ctenophore Beroe abyssicola: a novel type of Ca(2+) -regulated photoprotein.Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited stateCalcium dependence of aequorin bioluminescence dissected by random mutagenesis.Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120.Ca2+-regulated photoproteins: effective immunoassay reporters.Retrospective on the development of aequorin and aequorin-based imaging to visualize changes in intracellular free [Ca(2+) ].Mutants of Ca2+ -regulated Photoprotein Obelin for Site-specific Conjugation.Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase.1001 lights: luciferins, luciferases, their mechanisms of action and applications in chemical analysis, biology and medicine.Fluorescent Protein-photoprotein Fusions and Their Applications in Calcium Imaging.QM/MM study on the light emitters of aequorin chemiluminescence, bioluminescence, and fluorescence: a general understanding of the bioluminescence of several marine organisms.Fluorescence properties of Ca2+-independent discharged obelin and its application prospects.Effects of alcohols on fluorescence intensity and color of a discharged-obelin-based biomarker.Spectral tuning of obelin bioluminescence by mutations of Trp92.Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence.Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein.Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes.The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding.Cloning, sequencing, expression and structural investigation of mnemiopsin from Mnemiopsis leidyi: an attempt toward understanding Ca2+-regulated photoproteins.Semisynthetic photoprotein reporters for tracking fast Ca(2+) transients.Bioluminescent properties of obelin and aequorin with novel coelenterazine analogues.Site-directed mutagenesis of photoprotein mnemiopsin: implication of some conserved residues in bioluminescence properties.Development of a Ca(2+)-activated photoprotein, Photina, and its application to high-throughput screening.Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+ -regulated Photoproteins of Different Organisms.Characterization of hydromedusan Ca(2+)-regulated photoproteins as a tool for measurement of Ca(2+)concentration.Effect of halogenated fluorescent compounds on bioluminescent reactions.Bioluminescent and spectroscopic properties of His-Trp-Tyr triad mutants of obelin and aequorin.Hydrogen-bond networks between the C-terminus and Arg from the first α-helix stabilize photoprotein molecules.
P2860
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P2860
Obelin from the bioluminescent marine hydroid Obelia geniculata: cloning, expression, and comparison of some properties with those of other Ca2+-regulated photoproteins.
description
2002 nî lūn-bûn
@nan
2002年の論文
@ja
2002年論文
@yue
2002年論文
@zh-hant
2002年論文
@zh-hk
2002年論文
@zh-mo
2002年論文
@zh-tw
2002年论文
@wuu
2002年论文
@zh
2002年论文
@zh-cn
name
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@en
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@nl
type
label
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@en
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@nl
prefLabel
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@en
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@nl
P2093
P356
P1433
P1476
Obelin from the bioluminescent ...... Ca2+-regulated photoproteins.
@en
P2093
Eugene S Vysotski
John R Blinks
Ludmila P Burakova
Svetlana V Markova
P304
P356
10.1021/BI0117910
P407
P577
2002-02-01T00:00:00Z