Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.
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Growth factor regulation of hyaluronan synthesis and degradation in human dermal fibroblasts: importance of hyaluronan for the mitogenic response of PDGF-BBDetection and quantification of gene expression in environmental bacteriologymRNA extraction and reverse transcription-PCR protocol for detection of nifH gene expression by Azotobacter vinelandii in soilRat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR greenPCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency.Microbial source tracking markers for detection of fecal contamination in environmental waters: relationships between pathogens and human health outcomesValidation of kinetics similarity in qPCRConidia of the insect pathogenic fungus, Metarhizium anisopliae, fail to adhere to mosquito larval cuticleGene expression analysis of rocket salad under pre-harvest and postharvest stresses: A transcriptomic resource for Diplotaxis tenuifolia.Single-Cell Isolation and Gene Analysis: Pitfalls and PossibilitiesGanjam virus/Nairobi sheep disease virus induces a pro-inflammatory response in infected sheep.The transcriptomic signature of fasting murine liver.Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data.V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice.Expression stability of commonly used reference genes in canine articular connective tissues.Fasting induces a biphasic adaptive metabolic response in murine small intestine.Statistical modelling of transcript profiles of differentially regulated genes.Quantitative analysis of hTERT mRNA levels in cells microdissected from cytological specimens.Quantitative RT-PCR gene expression analysis of laser microdissected tissue samplesGeneration and characterization of human insulin-releasing cell lines.Selective control of primer usage in multiplex one-step reverse transcription PCRValidation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots.Glutamine synthetase in muscle is required for glutamine production during fasting and extrahepatic ammonia detoxification.Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol.Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNASensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp.Aestivation and hypoxia-related events share common silent neuron trafficking processes.Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimenThe identification of gut neuroendocrine tumor disease by multiple synchronous transcript analysis in bloodEstablishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene.miR-367 promotes epithelial-to-mesenchymal transition and invasion of pancreatic ductal adenocarcinoma cells by targeting the Smad7-TGF-β signalling pathway.Novel molecular method for detection of bovine-specific central nervous system tissues as bovine spongiform encephalopathy risk material in meat and meat products.Pitfalls of quantitative real-time reverse-transcription polymerase chain reactionHow are genes measured? Examples from studies on iron metabolism in pregnancy.A De novo Transcriptomic Approach to Identify Flavonoids and Anthocyanins "Switch-Off" in Olive (Olea europaea L.) Drupes at Different Stages of Maturation.Molecular methods for diagnosis of viral encephalitisThe microRNA miR-181c controls microglia-mediated neuronal apoptosis by suppressing tumor necrosis factor.Iron-mediated inhibition of mitochondrial manganese uptake mediates mitochondrial dysfunction in a mouse model of hemochromatosis.Conjugated linoleic acid isomers and their precursor fatty acids regulate peroxisome proliferator-activated receptor subtypes and major peroxisome proliferator responsive element-bearing target genes in HepG2 cell model.
P2860
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P2860
Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.
description
2002 nî lūn-bûn
@nan
2002年の論文
@ja
2002年学术文章
@wuu
2002年学术文章
@zh-cn
2002年学术文章
@zh-hans
2002年学术文章
@zh-my
2002年学术文章
@zh-sg
2002年學術文章
@yue
2002年學術文章
@zh
2002年學術文章
@zh-hant
name
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@en
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@nl
type
label
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@en
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@nl
prefLabel
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@en
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@nl
P2093
P1476
Sensitivity and accuracy of qu ...... on cDNA synthesis conditions.
@en
P2093
Antoon F M Moorman
Arnoud C Fijnvandraat
Ronald H Lekanne Deprez
P356
10.1016/S0003-2697(02)00021-0
P407
P577
2002-08-01T00:00:00Z