Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
about
Exploring protein fitness landscapes by directed evolutionFunctional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substratesStructure, electronic properties and catalytic behaviour of an activity-enhancing CYP102A1 (P450(BM3)) variantEvolving P450pyr hydroxylase for highly enantioselective hydroxylation at non-activated carbon atomUnusual Spectroscopic and Ligand Binding Properties of the Cytochrome P450-Flavodoxin Fusion Enzyme XplAKey Mutations Alter the Cytochrome P450 BM3 Conformational Landscape and Remove Inherent Substrate BiasUse of chemical auxiliaries to control p450 enzymes for predictable oxidations at unactivated C-h bonds of substratesVariations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamilyMicrobial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?Rational redesign of glucose oxidase for improved catalytic function and stabilityEngineering cytochrome P450 biocatalysts for biotechnology, medicine and bioremediationEvolutionary history of a specialized p450 propane monooxygenaseOlefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 EnzymesNeutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Identifying functionally important mutations from phenotypically diverse sequence data.Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3.Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds.Screening mutant libraries of fungal laccases in the presence of organic solvents.Tuning genetic control through promoter engineeringLaboratory-directed protein evolutionAnalysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis.Laboratory evolution of P450 BM-3 for mediated electron transfer.Streptomyces coelicolor A3(2) CYP102 protein, a novel fatty acid hydroxylase encoded as a heme domain without an N-terminal redox partnerBiocatalytic production of perillyl alcohol from limonene by using a novel Mycobacterium sp. cytochrome P450 alkane hydroxylase expressed in Pseudomonas putidaEnhancing the efficiency and regioselectivity of P450 oxidation catalysts by unnatural amino acid mutagenesis.A single active site mutation inverts stereoselectivity of 16-hydroxylation of testosterone catalyzed by engineered cytochrome P450 BM3.Directed evolution of the peroxidase activity of a de novo-designed protein.MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes.Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine.Directed evolution of DNA polymerases: construction and screening of DNA polymerase mutant libraries.The bioinorganic chemistry of iron in oxygenases and supramolecular assemblies.CYP153A6, a soluble P450 oxygenase catalyzing terminal-alkane hydroxylation.A panel of cytochrome P450 BM3 variants to produce drug metabolites and diversify lead compounds.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesisRapid and quantitative measurement of metabolic stability without chromatography or mass spectrometryAlkane hydroxylase genes in psychrophile genomes and the potential for cold active catalysis.Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamide.Evaluation and directed evolution for thermostability improvement of a GH 13 thermostable α-glucosidase from Thermus thermophilus TC11.
P2860
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P2860
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
description
2002 nî lūn-bûn
@nan
2002年の論文
@ja
2002年学术文章
@wuu
2002年学术文章
@zh
2002年学术文章
@zh-cn
2002年学术文章
@zh-hans
2002年学术文章
@zh-my
2002年学术文章
@zh-sg
2002年學術文章
@yue
2002年學術文章
@zh-hant
name
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@en
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@nl
type
label
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@en
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@nl
prefLabel
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@en
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@nl
P50
P356
P1433
P1476
Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
@en
P2888
P304
P356
10.1038/NBT744
P577
2002-10-07T00:00:00Z
P5875
P6179
1002614800