Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
about
Exploring protein fitness landscapes by directed evolutionOptimization of the bacterial cytochrome P450 BM3 system for the production of human drug metabolitesA Single Active-Site Mutation of P450BM-3 Dramatically Enhances Substrate Binding and Rate of Product FormationThe crystal structure of the FAD/NADPH-binding domain of flavocytochrome P450 BM3Evolving P450pyr hydroxylase for highly enantioselective hydroxylation at non-activated carbon atomKey Mutations Alter the Cytochrome P450 BM3 Conformational Landscape and Remove Inherent Substrate BiasStructure-Guided Directed Evolution of Highly Selective P450-Based Magnetic Resonance Imaging Sensors for Dopamine and SerotoninStructural evidence: a single charged residue affects substrate binding in cytochrome P450 BM-3Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamilyEngineering cytochrome P450 biocatalysts for biotechnology, medicine and bioremediationEvolutionary history of a specialized p450 propane monooxygenaseOlefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 EnzymesNeutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.Predicting oligonucleotide-directed mutagenesis failures in protein engineering.Laboratory-directed protein evolutionExpansion of substrate specificity of cytochrome P450 2A6 by random and site-directed mutagenesis.Laboratory evolution of P450 BM-3 for mediated electron transfer.Directed evolution: an approach to engineer enzymes.Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes.Application of a fluorescence-based continuous-flow bioassay to screen for diversity of cytochrome P450 BM3 mutant libraries.One-bead-one-catalyst approach to aspartic acid-based oxidation catalyst discoveryCharacterization of Cupriavidus metallidurans CYP116B1--a thiocarbamate herbicide oxygenating P450-phthalate dioxygenase reductase fusion protein.MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes.Enzyme-controlled nitrogen-atom transfer enables regiodivergent C-H amination.Replacement of natural cofactors by selected hydrogen peroxide donors or organic peroxides results in improved activity for CYP3A4 and CYP2D6.CYP153A6, a soluble P450 oxygenase catalyzing terminal-alkane hydroxylation.Stereoselective epoxidation of the last double bond of polyunsaturated fatty acids by human cytochromes P450.A panel of cytochrome P450 BM3 variants to produce drug metabolites and diversify lead compounds.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium.Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesisRapid and quantitative measurement of metabolic stability without chromatography or mass spectrometryIdentification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase.Combining substrate dynamics, binding statistics, and energy barriers to rationalize regioselective hydroxylation of octane and lauric acid by CYP102A1 and mutantsSelective hydroxylation of alkanes by an extracellular fungal peroxygenase.Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3.Cytochrome P450/redox partner fusion enzymes: biotechnological and toxicological prospects.Direct functionalization of nitrogen heterocycles via Rh-catalyzed C-H bond activationReplacement of the axial histidine heme ligand with cysteine in nitrophorin 1: spectroscopic and crystallographic characterization.P450-catalyzed intramolecular sp(3) C-H amination with arylsulfonyl azide substrates.
P2860
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P2860
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
description
2003 nî lūn-bûn
@nan
2003年の論文
@ja
2003年学术文章
@wuu
2003年学术文章
@zh
2003年学术文章
@zh-cn
2003年学术文章
@zh-hans
2003年学术文章
@zh-my
2003年学术文章
@zh-sg
2003年學術文章
@yue
2003年學術文章
@zh-hant
name
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@en
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@nl
type
label
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@en
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@nl
prefLabel
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@en
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@nl
P356
P1476
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.
@en
P2093
Matthew W Peters
Peter Meinhold
P304
13442-13450
P356
10.1021/JA0303790
P407
P577
2003-11-01T00:00:00Z