Site-directed mutagenesis of colicin E1 provides specific attachment sites for spin labels whose spectra are sensitive to local conformation.
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Analysis of the Intrinsically Disordered N-Terminus of the DNA Junction-Resolving Enzyme T7 Endonuclease I: Identification of Structure Formed upon DNA BindingCombining crystallography and EPR: crystal and solution structures of the multidomain cochaperone DnaJGenetically engineered metal ion binding sites on the outside of a Channel's transmembrane beta-barrel.Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: applications to soluble and transmembrane receptors of bacterial chemotaxis.High-field EPR spectroscopy applied to biological systems: characterization of molecular switches for electron and ion transfer.Recent excitements in protein NMR: Large proteins and biologically relevant dynamics.Probing the structural basis and adsorption mechanism of an enzyme on nano-sized protein carriers.Calculation of electron paramagnetic resonance spectra from Brownian dynamics trajectories: application to nitroxide side chains in proteinsStructure and dynamics of an imidazoline nitroxide side chain with strongly hindered internal motion in proteins.A method for distance determination in proteins using a designed metal ion binding site and site-directed spin labeling: evaluation with T4 lysozymeThermal motions of surface alpha-helices in the D-galactose chemosensory receptor. Detection by disulfide trappingIdentification of cystic fibrosis transmembrane conductance regulator channel-lining residues in and flanking the M6 membrane-spanning segment.A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data.Characterization of a folding intermediate of human carbonic anhydrase II: probing local mobility by electron paramagnetic resonance.Determination of electrostatic potentials at biological interfaces using electron-electron double resonance.Visualizing transient dark states by NMR spectroscopy.Structure and dynamics of a conformationally constrained nitroxide side chain and applications in EPR spectroscopy.Reaction of diphtheria toxin channels with sulfhydryl-specific reagents: observation of chemical reactions at the single molecule level.A cysteine residue in the third membrane-spanning segment of the human D2 dopamine receptor is exposed in the binding-site crevice.Pulsed ESR dipolar spectroscopy for distance measurements in immobilized spin labeled proteins in liquid solution.Cocaine alters the accessibility of endogenous cysteines in putative extracellular and intracellular loops of the human dopamine transporter.Protein global fold determination using site-directed spin and isotope labeling.(19)F Paramagnetic Relaxation Enhancement: A Valuable Tool for Distance Measurements in ProteinsLocations of Arg-82, Asp-85, and Asp-96 in helix C of bacteriorhodopsin relative to the aqueous boundariesThe spin label amino acid TOAC and its uses in studies of peptides: chemical, physicochemical, spectroscopic, and conformational aspects.Structure in the channel forming domain of colicin E1 bound to membranes: the 402-424 sequence.EPR relaxation-enhancement-based distance measurements on orthogonally spin-labeled T4-lysozyme.A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T1 relaxation enhancement.Structure and dynamics of Escherichia coli chemosensory receptors. Engineered sulfhydryl studies.Broadband inversion PELDOR spectroscopy with partially adiabatic shaped pulses.Colicin E1 binding to membranes: time-resolved studies of spin-labeled mutants.Electron spin-labelling of the EutC subunit in B12-dependent ethanolamine ammonia-lyase reveals dynamics and a two-state conformational equilibrium in the N-terminal, signal-sequence-associated domain.Gi- and Gs-coupled GPCRs show different modes of G-protein binding.Nitroxyl radicals for studying electron transfer.
P2860
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P2860
Site-directed mutagenesis of colicin E1 provides specific attachment sites for spin labels whose spectra are sensitive to local conformation.
description
1989 nî lūn-bûn
@nan
1989年の論文
@ja
1989年学术文章
@wuu
1989年学术文章
@zh
1989年学术文章
@zh-cn
1989年学术文章
@zh-hans
1989年学术文章
@zh-my
1989年学术文章
@zh-sg
1989年學術文章
@yue
1989年學術文章
@zh-hant
name
Site-directed mutagenesis of c ...... nsitive to local conformation.
@en
Site-directed mutagenesis of c ...... nsitive to local conformation.
@nl
type
label
Site-directed mutagenesis of c ...... nsitive to local conformation.
@en
Site-directed mutagenesis of c ...... nsitive to local conformation.
@nl
prefLabel
Site-directed mutagenesis of c ...... nsitive to local conformation.
@en
Site-directed mutagenesis of c ...... nsitive to local conformation.
@nl
P2093
P356
P1433
P1476
Site-directed mutagenesis of c ...... nsitive to local conformation.
@en
P2093
P2860
P304
P356
10.1002/PROT.340060312
P407
P577
1989-01-01T00:00:00Z