Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining.

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining.

Item
http://www.wikidata.org/entity/Q50484094
A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cellsBiomimetic postcapillary expansions for enhancing rare blood cell separation on a microfluidic chip.Apparent mtDNA sequence heterogeneity in single human blood CD34+ cells is markedly affected by storage and transportSuccessful mobilization, intra-apheresis recruitment, and harvest of hematopoietic progenitor cells by addition of plerixafor and subsequent large-volume leukapheresis.Clinical grade adult stem cell bankingQuality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation.Flow cytometric analyses of CD34+ cells with inclusion of internal positive controls.Determining post-thaw CD34+ cell dose of cryopreserved haematopoietic progenitor cells demonstrates high recovery and confirms their integrity.Optimization of the freezing process for hematopoietic progenitor cells: effect of precooling, initial dimethyl sulfoxide concentration, freezing program, and storage in vapor-phase or liquid nitrogen on in vitro white blood cell quality.Assessment of cell viability, early apoptosis, and hematopoietic potential in umbilical cord blood units after storage.Cryopreservation of cellular products in a closed-bag system with an incorporated dimethyl sulfoxide-resistant sterile filter outside of cleanroom facilities.
P2860

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining.

Item
http://www.wikidata.org/entity/Q50484094
description
2005 nî lūn-bûn
@nan
2005年の論文
@ja
2005年学术文章
@wuu
2005年学术文章
@zh
2005年学术文章
@zh-cn
2005年学术文章
@zh-hans
2005年学术文章
@zh-my
2005年学术文章
@zh-sg
2005年學術文章
@yue
2005年學術文章
@zh-hant
name
Establishment and optimization ...... rypan blue exclusion staining.
@en
Establishment and optimization ...... rypan blue exclusion staining.
@nl
type
label
Establishment and optimization ...... rypan blue exclusion staining.
@en
Establishment and optimization ...... rypan blue exclusion staining.
@nl
prefLabel
Establishment and optimization ...... rypan blue exclusion staining.
@en
Establishment and optimization ...... rypan blue exclusion staining.
@nl
P1433
P1476
P2093
P2860
P304
P31
P356
P433
P478
P577
P698
P921
P356
P1433
P1476
Establishment and optimization ...... rypan blue exclusion staining.
@en
P2093
P2860
P304
P31
P356
10.1111/J.1537-2995.2005.00174.X
P433
P478
P577
P698
P921