High-precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion protein.
about
Wide-Field Multi-Parameter FLIM: long-term minimal invasive observation of proteins in living cellsFolding study of Venus reveals a strong ion dependence of its yellow fluorescence under mildly acidic conditions.Quantitative analysis of energy transfer between fluorescent proteins in CFP-GBP-YFP and its response to Ca2+.The fluorescent protein palette: tools for cellular imaging.Fiber laser based two-photon FRET measurement of calmodulin and mCherry-E(0)GFP proteins.Partial acceptor photobleaching-based quantitative FRET method completely overcoming emission spectral crosstalks.Fluorescence lifetime readouts of Troponin-C-based calcium FRET sensors: a quantitative comparison of CFP and mTFP1 as donor fluorophores.High throughput FRET analysis of protein-protein interactions by slide-based imaging laser scanning cytometry.A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowthCorrelating calcium binding, Förster resonance energy transfer, and conformational change in the biosensor TN-XXL.Methodological considerations for global analysis of cellular FLIM/FRET measurements.Phase differential enhancement of FLIM to distinguish FRET components of a biosensor for monitoring molecular activity of Membrane Type 1 Matrix Metalloproteinase in live cells.Live-Cell imaging and measurement of intracellular pH in filamentous fungi using a genetically encoded ratiometric probeNoninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometry.Investigating State Restriction in Fluorescent Protein FRET Using Time-Resolved Fluorescence and AnisotropyIn-depth fluorescence lifetime imaging analysis revealing SNAP25A-Rabphilin 3A interactions.Structural changes of yellow Cameleon domains observed by quantitative FRET analysis and polarized fluorescence correlation spectroscopy.Fusion of GFP to the M.EcoKI DNA methyltransferase produces a new probe of Type I DNA restriction and modification enzymes.PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroidesInteraction of proteins associated with the magnetosome assembly in magnetotactic bacteria as revealed by two-hybrid two-photon excitation fluorescence lifetime imaging microscopy Förster resonance energy transferEnzyme-Triggered Dissociation of a FRET-Based Protein Biosensor Monitored by Synchrotron SAXS.Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy technique.T cell activation results in conformational changes in the Src family kinase Lck to induce its activation.A quantitative protocol for dynamic measurements of protein interactions by Forster resonance energy transfer-sensitized fluorescence emission
P2860
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P2860
High-precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion protein.
description
2007 nî lūn-bûn
@nan
2007年の論文
@ja
2007年学术文章
@wuu
2007年学术文章
@zh
2007年学术文章
@zh-cn
2007年学术文章
@zh-hans
2007年学术文章
@zh-my
2007年学术文章
@zh-sg
2007年學術文章
@yue
2007年學術文章
@zh-hant
name
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@en
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@nl
type
label
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@en
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@nl
prefLabel
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@en
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@nl
P2093
P50
P1476
High-precision FLIM-FRET in fi ...... simple CFP-YFP fusion protein.
@en
P2093
Anita C Jones
David T F Dryden
G Joan Grindlay
Kirsten Altenbach
Michael Millington
Mojca Bencina
Steven W Magennis
P304
P356
10.1016/J.BPC.2007.01.008
P577
2007-02-01T00:00:00Z