about
A fluorescent cassette-based strategy for engineering multiple domain fusion proteinsSoluble expression of recombinant proteins in the cytoplasm of Escherichia coliDirected evolution of single-chain Fv for cytoplasmic expression using the beta-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregationDesign, expression, and purification of a Flaviviridae polymerase using a high-throughput approach to facilitate crystal structure determinationSplit green fluorescent protein as a modular binding partner for protein crystallizationEffects of protein engineering and rational mutagenesis on crystal lattice of single chain antibody fragmentsAutomated main-chain model building by template matching and iterative fragment extensionCrystal structure of the RUN domain of the RAP2-interacting protein xBenefits of structural genomics for drug discovery researchStructural and biophysical characterization of an epitope-specific engineered Fab fragment and complexation with membrane proteins: implications for co-crystallization.Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation.Application of protein engineering to enhance crystallizability and improve crystal properties.Engineering membrane protein overproduction in Escherichia coli.Resurrecting abandoned proteins with pure water: CD and NMR studies of protein fragments solubilized in salt-free water.Combinatorial approaches to novel proteins.Combinatorial Domain Hunting: An effective approach for the identification of soluble protein domains adaptable to high-throughput applications.New molecular reporters for rapid protein folding assaysInsight into "insoluble proteins" with pure water.Conversion of scFv peptide-binding specificity for crystal chaperone development.DNA shuffling as a tool for protein crystallizationA high-throughput immobilized bead screen for stable proteins and multi-protein complexes.Experimental mapping of soluble protein domains using a hierarchical approachCombining computational and experimental screening for rapid optimization of protein propertiesGFP facilitates native purification of recombinant perlucin derivatives and delays the precipitation of calcium carbonateEngineering and characterization of a superfolder green fluorescent protein.Prediction and analysis of protein solubility using a novel scoring card method with dipeptide compositionMulticolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexesRational improvement of simvastatin synthase solubility in Escherichia coli leads to higher whole-cell biocatalytic activity.Highly diverse protein library based on the ubiquitous (β/α)₈ enzyme fold yields well-structured proteins through in vitro folding selection.Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli.Structural proteomics in drug discovery.Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum.Understanding the relationship between the primary structure of proteins and its propensity to be soluble on overexpression in Escherichia coli.Periscope: quantitative prediction of soluble protein expression in the periplasm of Escherichia coli.The rough energy landscape of superfolder GFP is linked to the chromophoreStatistical density modification using local pattern matching.Automated side-chain model building and sequence assignment by template matchingImproving macromolecular atomic models at moderate resolution by automated iterative model building, statistical density modification and refinementAutomation of protein purification for structural genomics.Chaperone discovery.
P2860
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P2860
description
2002 nî lūn-bûn
@nan
2002年の論文
@ja
2002年学术文章
@wuu
2002年学术文章
@zh
2002年学术文章
@zh-cn
2002年学术文章
@zh-hans
2002年学术文章
@zh-my
2002年学术文章
@zh-sg
2002年學術文章
@yue
2002年學術文章
@zh-hant
name
Engineering soluble proteins for structural genomics.
@en
Engineering soluble proteins for structural genomics.
@nl
type
label
Engineering soluble proteins for structural genomics.
@en
Engineering soluble proteins for structural genomics.
@nl
prefLabel
Engineering soluble proteins for structural genomics.
@en
Engineering soluble proteins for structural genomics.
@nl
P2093
P2860
P356
P1433
P1476
Engineering soluble proteins for structural genomics.
@en
P2093
Beom-Seop Rho
Chang-Yub Kim
Elaine C Liong
Emily Piltch
Geoffrey S Waldo
Jean-Denis Pédelacq
Joel Berendzen
Min S Park
Thomas C Terwilliger
P2860
P2888
P304
P356
10.1038/NBT732
P577
2002-08-19T00:00:00Z