A single-step transconjugation system for the introduction of unmarked deletions into Actinobacillus pleuropneumoniae serotype 7 using a sucrose sensitivity marker.
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Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia colimalT knockout mutation invokes a stringent type gene-expression profile in Actinobacillus pleuropneumoniae in bronchoalveolar fluid.Enzymes involved in anaerobic respiration appear to play a role in Actinobacillus pleuropneumoniae virulenceDevelopment of a markerless knockout method for Actinobacillus succinogenes.Pyridoxal phosphate synthases PdxS/PdxT are required for Actinobacillus pleuropneumoniae viability, stress tolerance and virulence.The SapA Protein Is Involved in Resistance to Antimicrobial Peptide PR-39 and Virulence of Actinobacillus pleuropneumoniaeThe ClpP protease is required for the stress tolerance and biofilm formation in Actinobacillus pleuropneumoniae.The generation of successive unmarked mutations and chromosomal insertion of heterologous genes in Actinobacillus pleuropneumoniae using natural transformationUse of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli.Immunization of pigs to prevent disease in humans: construction and protective efficacy of a Salmonella enterica serovar Typhimurium live negative-marker vaccineAttenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge.Analysis of the Actinobacillus pleuropneumoniae ArcA regulon identifies fumarate reductase as a determinant of virulenceProtection of piglets by a Haemophilus parasuis ghost vaccine against homologous challenge.Cloning and characterization of the Actinobacillus pleuropneumoniae fur gene and its role in regulation of ApxI and AfuABC expression.Actinobacillus pleuropneumoniae iron transport: a set of exbBD genes is transcriptionally linked to the tbpB gene and required for utilization of transferrin-bound iron.Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response.Construction of an Actinobacillus pleuropneumoniae serotype 2 prototype live negative-marker vaccine.fhuA of Actinobacillus pleuropneumoniae encodes a ferrichrome receptor but is not regulated by iron.Trimeric autotransporter adhesins contribute to Actinobacillus pleuropneumoniae pathogenicity in mice and regulate bacterial gene expression during interactions between bacteria and porcine primary alveolar macrophages.Identification of dimethyl sulfoxide reductase in Actinobacillus pleuropneumoniae and its role in infection.Attachment of Actinobacillus suis H91-0380 and Its Isogenic Adhesin Mutants to Extracellular Matrix Components of the Tonsils of the Soft Palate of Swine.Use of an Actinobacillus pleuropneumoniae multiple mutant as a vaccine that allows differentiation of vaccinated and infected animals.Deletion of the anaerobic regulator HlyX causes reduced colonization and persistence of Actinobacillus pleuropneumoniae in the porcine respiratory tract.Deletion of the ferric uptake regulator Fur impairs the in vitro growth and virulence of Actinobacillus pleuropneumoniae.Update on Actinobacillus pleuropneumoniae-knowledge, gaps and challenges.A streptomycin resistance marker in H. parasuis based on site-directed mutations in rpsL gene to perform unmarked in-frame mutations and to verify natural transformation.
P2860
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P2860
A single-step transconjugation system for the introduction of unmarked deletions into Actinobacillus pleuropneumoniae serotype 7 using a sucrose sensitivity marker.
description
1999 nî lūn-bûn
@nan
1999年の論文
@ja
1999年学术文章
@wuu
1999年学术文章
@zh-cn
1999年学术文章
@zh-hans
1999年学术文章
@zh-my
1999年学术文章
@zh-sg
1999年學術文章
@yue
1999年學術文章
@zh
1999年學術文章
@zh-hant
name
A single-step transconjugation ...... a sucrose sensitivity marker.
@en
A single-step transconjugation ...... a sucrose sensitivity marker.
@nl
type
label
A single-step transconjugation ...... a sucrose sensitivity marker.
@en
A single-step transconjugation ...... a sucrose sensitivity marker.
@nl
prefLabel
A single-step transconjugation ...... a sucrose sensitivity marker.
@en
A single-step transconjugation ...... a sucrose sensitivity marker.
@nl
P2093
P2860
P1476
A single-step transconjugation ...... a sucrose sensitivity marker.
@en
P2093
P2860
P304
P356
10.1111/J.1574-6968.1999.TB08721.X
P577
1999-10-01T00:00:00Z