Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression.
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Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ.Structure and function of FusB: an elongation factor G-binding fusidic acid resistance protein active in ribosomal translocation and recyclingThe extracellular signal-regulated kinase 3 (mitogen-activated protein kinase 6 [MAPK6])-MAPK-activated protein kinase 5 signaling complex regulates septin function and dendrite morphologyLOV Histidine Kinase Modulates the General Stress Response System and Affects the virB Operon Expression in Brucella abortusA Supercomplex Spanning the Inner and Outer Membranes Mediates the Biogenesis of β-Barrel Outer Membrane Proteins in Bacteria.Sequence Identification, Recombinant Production, and Analysis of the Self-Assembly of Egg Stalk Silk Proteins from Lacewing Chrysoperla carnea.Exploring ORFan domains in giant viruses: structure of mimivirus sulfhydryl oxidase R596A Novel Allosteric Mechanism of NF-κB Dimerization and DNA Binding Targeted by an Anti-Inflammatory Drug.Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.Leveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiaeA new method to customize protein expression vectors for fast, efficient and background free parallel cloning.A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substratesA practical comparison of ligation-independent cloning techniquesInfluenza A virus encoding secreted Gaussia luciferase as useful tool to analyze viral replication and its inhibition by antiviral compounds and cellular proteins.Eisosomes are dynamic plasma membrane domains showing pil1-lsp1 heteroligomer binding equilibrium.Cold shock induction of recombinant Arctic environmental genes.One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.A high-throughput expression screening platform to optimize the production of antimicrobial peptidesDNA-conjugated gold nanoparticles based colorimetric assay to assess helicase activity: a novel route to screen potential helicase inhibitorsEngineering Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase for Fully Active Amidated NAD Biosynthesis.Promoter swapping unveils the role of the Citrobacter rodentium CTS1 type VI secretion system in interbacterial competition.Structural and biochemical characterization of an RNA/DNA binding motif in the N-terminal domain of RecQ4 helicases.Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosomeCRISPR RNA-guided FokI nucleases repair a PAH variant in a phenylketonuria model.Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action.Generation of a vector suite for protein solubility screening.OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments.A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement.Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: advantages of high-throughput screening.DTIE, a novel core promoter element that directs start site selection in TATA-less genes.A versatile vector for mycobacterial protein production with a functional minimized acetamidase regulon.A Metabolic Gene Cluster in the Wheat W1 and the Barley Cer-cqu Loci Determines β-Diketone Biosynthesis and Glaucousness.The Human RecQ4 Helicase Contains a Functional RecQ C-terminal Region (RQC) That Is Essential for Activity.TM7SF3, a novel p53-regulated homeostatic factor, attenuates cellular stress and the subsequent induction of the unfolded protein response.The vapB-vapC Operon of Acidovorax citrulli Functions as a Bona-fide Toxin-Antitoxin ModuleComparative genetics. Systematic discovery of cap-independent translation sequences in human and viral genomes.The Drosophila formin Fhos is a primary mediator of sarcomeric thin-filament array assembly.Functionally Active Fc Mutant Antibodies Recognizing Cancer Antigens Generated Rapidly at High Yields.Do Src Kinase and Caveolin Interact Directly with Na,K-ATPase?Thermodynamic Protein Destabilization by GFP Tagging: A Case of Interdomain Allostery.
P2860
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P2860
Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression.
description
2010 nî lūn-bûn
@nan
2010年の論文
@ja
2010年学术文章
@wuu
2010年学术文章
@zh-cn
2010年学术文章
@zh-hans
2010年学术文章
@zh-my
2010年学术文章
@zh-sg
2010年學術文章
@yue
2010年學術文章
@zh
2010年學術文章
@zh-hant
name
Applications of the Restrictio ...... ations and protein expression.
@en
Applications of the Restriction Free
@nl
type
label
Applications of the Restrictio ...... ations and protein expression.
@en
Applications of the Restriction Free
@nl
prefLabel
Applications of the Restrictio ...... ations and protein expression.
@en
Applications of the Restriction Free
@nl
P2093
P1476
Applications of the Restrictio ...... ations and protein expression.
@en
P2093
Ada Dantes
Reut Bernheim
Tamar Unger
Yoav Peleg
Yossi Jacobovitch
P356
10.1016/J.JSB.2010.06.016
P577
2010-06-25T00:00:00Z