Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and thawing.
about
Structure of a protein G helix variant suggests the importance of helix propensity and helix dipole interactions in protein designSpecificity determinants for lipids bound to beta-barrel proteinsStructure and dynamics of de novo proteins from a designed superfamily of 4-helix bundlesIron-regulated transcription of the pvdA gene in Pseudomonas aeruginosa: effect of Fur and PvdS on promoter activityMutagenesis-based definitions and probes of residue burial in proteins.An improved optical tweezers assay for measuring the force generation of single kinesin molecules.2D 1H and 3D 1H-15N NMR of zinc-rubredoxins: contributions of the beta-sheet to thermostability.The NMR structure of a stable and compact all-beta-sheet variant of intestinal fatty acid-binding proteinStably folded de novo proteins from a designed combinatorial library.Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937De novo heme proteins from designed combinatorial librariesPeroxidase activity of de novo heme proteins immobilized on electrodes.Stereochemical criteria for prediction of the effects of proline mutations on protein stability.Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin.Binding of small molecules to cavity forming mutants of a de novo designed protein.The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937.RegA proteins from phage T4 and RB69 have conserved helix-loop groove RNA binding motifs but different RNA binding specificities.Preparation of Soluble Proteins from Escherichia coli.PaeX, a second pectin acetylesterase of Erwinia chrysanthemi 3937.Statistical experimental design guided optimization of a one-pot biphasic multienzyme total synthesis of amorpha-4,11-diene.Molecular cloning and characterization of Coccidioides immitis antigen 2 cDNA.Overview of the purification of recombinant proteins.A rubrerythrin operon and nigerythrin gene in Desulfovibrio vulgaris (Hildenborough).Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family.Expression of a codon-optimised recombinant Ara h 2.02 peanut allergen in Escherichia coli.Construction and Periplasmic Expression of the Anti-EGFRvIII ScFv Antibody Gene in Escherichia coli.Intrinsic Disorder of the C-Terminal Domain of Drosophila Methoprene-Tolerant Protein.KPC-9, a novel carbapenemase from clinical specimens in IsraelA de novo designed protein protein interface.Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant.Molecular cloning and photoperiod-regulated expression of gibberellin 20-oxidase from the long-day plant spinach.Systematic Perturbations of Binuclear Non-heme Iron Sites: Structure and Dioxygen Reactivity of de Novo Due Ferri Proteins.Cofactor binding and enzymatic activity in an unevolved superfamily of de novo designed 4-helix bundle proteins.Evaluation of an Albumin-Binding Domain Protein Fused to Recombinant Human IL-2 and Its Effects on the Bioactivity and Serum Half-Life of the Cytokine.Structure-function relationships in staphylokinase as revealed by "clustered charge to alanine" mutagenesis.DNA distortion mechanism for transcriptional activation by ZntR, a Zn(II)-responsive MerR homologue in Escherichia coli.Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells.Alteration of the oxygen-dependent reactivity of de novo Due Ferri proteins.Kinetics of infected insect cell osmolysis and enhanced protein release using a modified disruption method.Leaderless polypeptides efficiently extracted from whole cells by osmotic shock.
P2860
Q27626281-24C7949E-16D0-4B6B-AF56-7A7E2965B8C1Q27642466-7BEA5B26-1337-408A-87C9-82415123B250Q27650455-2AEAD82E-A211-4584-907A-0FE07922CD21Q28493094-282B05ED-888F-4BB1-BC77-1D68BA6C44E2Q30351711-C8BEEC42-F60F-46EA-9E49-7166ED7CA8D8Q30423655-4B35A735-1355-4D67-851F-6F23B1C2BE4EQ30424602-30E55C2F-C84D-4909-A50B-288F3C7FF5B7Q30482655-47DE3CAE-16A4-47C9-B000-79BA40212E9AQ30876837-7534AB95-99F0-48EF-9FCF-F3FE344049C7Q31214629-D572C4E2-40BF-4BD9-AA63-887D41EEACEFQ32161504-1F8FA561-A7D0-4207-9DAD-AC311D874989Q33296366-2C733739-3563-4A64-B169-3DDAB0BFE200Q33309456-C647F4F4-C099-41A4-B76B-B8A54F499941Q33789393-BF31E4B9-9834-4537-BEE4-C4A71A055E04Q33822757-79FBB607-6AD7-4649-B3F0-9ADBC69AE021Q33992419-0662CB58-56D0-462A-9B8C-F45D9586A350Q34278307-CC115BB4-CFE1-4CD2-932C-78C5C87331DDQ34743490-B20B5412-0EBA-44E5-8F60-396C09C7338BQ34977492-58A7A3C5-A3A4-49DB-BCEA-9EAFFD8E73F1Q35053195-DBD1A151-AE7A-4117-B27F-C696367A3277Q35505628-8B4F98B7-916A-4B9D-B451-E74015392063Q35541296-0A6BAE5D-6C37-4898-ADEC-53774DD00503Q35626546-CA08DC01-4D78-4F26-A8CA-88858F56E871Q35632982-F75224A8-23D0-4899-9C82-05932FA5FCA7Q35789754-60EAE8AA-C230-4831-9BA9-7E3DD0894832Q35997951-352992A6-3915-4A3B-A9C4-CB0F87EA0C67Q36140744-78939FB5-86AB-4828-B34B-7BA967E055C8Q36364179-1F875302-EEA3-4695-A97E-421FF8B4E6A2Q36420294-A871CAAE-34CE-42D2-9503-2BA7A9F4D3A9Q36476973-683E71C0-189C-4A36-92F4-67887C02774CQ36811549-D6F9C717-E11E-4AC9-944C-E661492B7D19Q36872282-06BC3D13-071B-4BC6-B296-A8B91F08FECEQ37416350-6C62B6FD-2CFD-4D53-9500-3BC1F0666FC6Q37611373-B958945C-6B27-4EFE-87D9-4081CE4DE343Q38289352-7D35CBBB-5673-4C8C-BAEC-1F01B05115A3Q38317199-61A858F8-1182-44D5-8D23-C9D9176ED5B8Q38809165-2211AE8D-FCC2-4A8E-A69D-288335D1DA8AQ39532378-244DD24A-F849-4DEA-ADBF-7A277F5A6DF2Q39584243-CC3174C1-339B-4240-872F-98A23C2C9D89Q39846925-D030549E-AD98-4AD9-AB47-539EED3057A7
P2860
Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and thawing.
description
1994 nî lūn-bûn
@nan
1994年の論文
@ja
1994年学术文章
@wuu
1994年学术文章
@zh-cn
1994年学术文章
@zh-hans
1994年学术文章
@zh-my
1994年学术文章
@zh-sg
1994年學術文章
@yue
1994年學術文章
@zh
1994年學術文章
@zh-hant
name
Recombinant proteins can be is ...... ycles of freezing and thawing.
@en
Recombinant proteins can be is ...... ycles of freezing and thawing.
@nl
type
label
Recombinant proteins can be is ...... ycles of freezing and thawing.
@en
Recombinant proteins can be is ...... ycles of freezing and thawing.
@nl
prefLabel
Recombinant proteins can be is ...... ycles of freezing and thawing.
@en
Recombinant proteins can be is ...... ycles of freezing and thawing.
@nl
P2860
P356
P1433
P1476
Recombinant proteins can be is ...... ycles of freezing and thawing.
@en
P2093
P2860
P304
P356
10.1038/NBT1294-1357
P577
1994-12-01T00:00:00Z