High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity
about
Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzymeVariants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele- and methylation-specific amplification.PCR performance of a thermostable heterodimeric archaeal DNA polymerase.Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems.Extensive recombination due to heteroduplexes generates large amounts of artificial gene fragments during PCRDirected evolution of polymerase function by compartmentalized self-replicationFluorescence correlation analysis of probe diffusion simplifies quantitative pathogen detection by PCR.Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site.Directed polymerase evolution.Nucleic acid amplification using microfluidic systems.Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA.Deoxy- and dideoxynucleotide discrimination and identification of critical 5' nuclease domain residues of the DNA polymerase I from Mycobacterium tuberculosis.Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA.Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates.DNA enzymology above 100 degrees C. Topoisomerase V unlinks circular DNA at 80-122 degrees C.DNA-dependent DNA polymerases.Global conformations, hydrodynamics, and X-ray scattering properties of Taq and Escherichia coli DNA polymerases in solution.Comparison of methods for quantification of subtle splice variants.Salt dependence of DNA binding by Thermus aquaticus and Escherichia coli DNA polymerases.Heterogeneity of internal tandem duplications in the c-kit of dogs with multiple mast cell tumours.Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets.Multiplex time-reducing quantitative polymerase chain reaction assay for determination of telomere length in blood and tissue DNA.Low fidelity mutants in the O-helix of Thermus aquaticus DNA polymerase I.A molecular approach to the taxonomy and biogeography of African parrots
P2860
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P2860
High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity
description
im Mai 1993 veröffentlichter wissenschaftlicher Artikel
@de
wetenschappelijk artikel
@nl
наукова стаття, опублікована в травні 1993
@uk
name
High-level expression, purific ...... 5' to 3' exonuclease activity
@en
High-level expression, purific ...... 5' to 3' exonuclease activity
@nl
type
label
High-level expression, purific ...... 5' to 3' exonuclease activity
@en
High-level expression, purific ...... 5' to 3' exonuclease activity
@nl
prefLabel
High-level expression, purific ...... 5' to 3' exonuclease activity
@en
High-level expression, purific ...... 5' to 3' exonuclease activity
@nl
P2093
P356
P1433
P1476
High-level expression, purific ...... 5' to 3' exonuclease activity
@en
P2093
D H Gelfand
F C Lawyer
P A Landre
R D Abramson
P304
P356
10.1101/GR.2.4.275
P577
1993-05-01T00:00:00Z