about
Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational designA Conserved Active-site Threonine Is Important for Both Sugar and Flavin Oxidations of Pyranose 2-OxidaseImportance of the gating segment in the substrate-recognition loop of pyranose 2-oxidaseH-bonding and positive charge at the N5/O4 locus are critical for covalent flavin attachment in trametes pyranose 2-oxidaseThe 1.6 Å Crystal Structure of Pyranose Dehydrogenase from Agaricus meleagris Rationalizes Substrate Specificity and Reveals a Flavin IntermediateEngineering of pyranose 2-oxidase: improvement for biofuel cell and food applications through semi-rational protein design.Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design.Thermostable variants of pyranose 2-oxidase showing altered substrate selectivity for glucose and galactose.A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenizationRecombinant horseradish peroxidase variants for targeted cancer treatment.A novel toolbox for E. coli lysis monitoring.Production strategies for active heme-containing peroxidases from E. coli inclusion bodies - a review.Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli.A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastorisThe E. coli pET expression system revisited-mechanistic correlation between glucose and lactose uptake.Characterisation of recombinant pyranose oxidase from the cultivated mycorrhizal basidiomycete Lyophyllum shimeji (hon-shimeji)A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli.Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter.A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastorisHeterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme.On-line multiple component analysis for efficient quantitative bioprocess development.Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli.A sensitive and robust HPLC method to quantify recombinant antibody fragments in E. coli crude cell lysate.Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae.The production of a recombinant tandem single chain fragment variable capable of binding prolamins triggering celiac disease.Lecithin is the key material attribute in soy bean oil affecting filamentous bioprocesses.Wanted: more monitoring and control during inclusion body processingE. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparisonKinetics and Predicted Structure of a Novel Xylose Reductase fromInclusion Body Bead Size in Controlled by Physiological FeedingImproving the Performance of Horseradish Peroxidase by Site-Directed MutagenesisRecombinant production of a hard-to-express membrane-bound cytochrome P450 in different yeasts-Comparison of physiology and productivityA fast and simple approach to optimize the unit operation high pressure homogenization - a case study for a soluble therapeutic protein in E. coliThe impact of technical failures during cultivation of an inclusion body processThe effect of amino acid supplementation in an industrial CHO processA defined cultivation medium for Sulfolobus acidocaldariusMonitoring and control strategies for inclusion body production in E. coli based on glycerol consumptionThe Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression SystemSpatially Resolved Effects of Protein Freeze-Thawing in a Small-Scale Model Using Monoclonal Antibodies
P50
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P50
description
onderzoeker
@nl
researcher, ORCID id # 0000-0003-0916-0644
@en
name
Oliver Spadiut
@ast
Oliver Spadiut
@en
Oliver Spadiut
@es
Oliver Spadiut
@nl
type
label
Oliver Spadiut
@ast
Oliver Spadiut
@en
Oliver Spadiut
@es
Oliver Spadiut
@nl
prefLabel
Oliver Spadiut
@ast
Oliver Spadiut
@en
Oliver Spadiut
@es
Oliver Spadiut
@nl
P106
P31
P496
0000-0003-0916-0644