The TUNEL assay consistently underestimates DNA damage in human spermatozoa and is influenced by DNA compaction and cell vitality: development of an improved methodology
about
Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertilityAre sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?Double stranded sperm DNA breaks, measured by Comet assay, are associated with unexplained recurrent miscarriage in couples without a female factorSperm DNA fragmentation and oxidation are independent of malondialdheyde.A simple sperm DNA toroid integrity test and risk of miscarriageMitochondrial permeability transition increases reactive oxygen species production and induces DNA fragmentation in human spermatozoa.Can apoptosis and necrosis coexist in ejaculated human spermatozoa during in vitro semen bacterial infection?Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage.Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase.Variation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques: A Possible New Male Predictive Parameter of Pregnancy?Mechanisms and clinical correlates of sperm DNA damage.Topology of chromosome centromeres in human sperm nuclei with high levels of DNA damage.Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenariosThe source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies.TUNEL labeling with BrdUTP/anti-BrdUTP greatly underestimates the level of sperm DNA fragmentation in semen evaluation.Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-β1-meidated ERK/JNK/NF-κB signaling pathways.Mitochondrial outer membrane permeabilization increases reactive oxygen species production and decreases mean sperm velocity but is not associated with DNA fragmentation in human sperm.Inter- and intra-laboratory standardization of TUNEL assay for assessment of sperm DNA fragmentation.When and how should new technology be introduced into the IVF laboratory?Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.Is the sperm DNA status the best predictor of both natural and assisted conception?Some relevant points on sperm DNA fragmentation tests.Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay.Analysis of the global methylation status of human spermatozoa and its association with the tendency of these cells to enter apoptosis.Aldehyde Dehydrogenase Plays a Pivotal Role in the Maintenance of Stallion Sperm Motility.Involvement of homocysteine, homocysteine thiolactone, and paraoxonase type 1 (PON-1) in the etiology of defective human sperm function.The paradoxical relationship between stallion fertility and oxidative stress.European Academy of Andrology revives the International Journal of Andrology Award and Lecture.The Simmet lecture: new horizons on an old landscape--oxidative stress, DNA damage and apoptosis in the male germ line.On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid.Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa.Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage.Processes involved in assisted reproduction technologies significantly increase sperm DNA fragmentation and phosphatidylserine translocation
P2860
Q24634091-9AABDC3E-AA09-4C59-8956-3DCCAA239737Q28087808-09F1E22A-1E74-432C-A4F8-EECAFE50527EQ34427240-C4FCC81A-C8EC-4855-9701-5834F8CA0459Q34992913-EA8F1DC5-6CCA-4B23-8D48-31B784E8E5B9Q35026532-C0BF7A32-1224-4F0C-A52A-B4FC52D85AEEQ35557888-57E826D8-B115-4793-B990-49EF081CF928Q35599571-881FDA56-3541-434D-AA17-9470644584FDQ35819883-29A0B952-9F77-4223-85C2-7AB8A6E76744Q36294679-953956AD-C060-48CB-908E-550375B0454DQ36993083-9D21ED97-C938-4F7B-B8B6-E5E347E9C5AFQ37074534-72E5F291-4742-4143-A1EF-77D3BBF4D99AQ37202548-B0226FA1-61E1-4ABB-9052-3028F243E3D2Q37531089-D9484654-F396-456C-BFEE-3E3E4B43DC84Q38095200-57DA4D37-9F18-4DCD-87C5-F69141D6E29DQ38645530-E1F4F06F-5FF6-4497-A4A6-B1437DB5CE01Q38717810-4326CC10-F606-4576-9D11-88426844FE6EQ38936950-FBDC5370-E425-45FD-8399-C3B1A2E58279Q38937254-EDAC8704-2FA2-4B00-B7F2-7B6C480343B4Q41606774-5841AE5F-F817-404A-9661-9D7C3658DB18Q42087926-43F3E7E8-C661-423F-BC52-A561F16061FAQ42625987-585E835B-6865-4AA6-A083-4DB4A1FAEB1AQ42693683-C3628CA0-1838-491D-B3D2-4B72F3197CAAQ44003953-A9428C0D-C22A-47A1-9665-D9FF10BBE05AQ45746565-C5F31474-C831-4E90-A728-F3CB7426B7F0Q46559905-3E82342D-8E91-4A5A-8AAA-3F5BC5271A74Q46603019-21549595-583C-493C-870A-B8A38A1DBA55Q46858780-D251E6BA-879A-4ABF-8A8A-4F1ABE311CE0Q48302814-DBC1D8F5-2BF8-4052-91E5-A2F2F9151B50Q48532344-DEA388C1-80AD-4088-B8BE-F36EDF22DC96Q50496912-CE3B7384-20B5-4DDA-80E6-FA0EA718C69DQ50634713-5677F113-DE09-4FCC-AE2F-7B01BBF8891EQ55236815-BBAC288C-93DE-4248-83A1-EE7DC74D4E99Q57095700-FBB8B1A3-82BD-43CD-835F-0690279D976F
P2860
The TUNEL assay consistently underestimates DNA damage in human spermatozoa and is influenced by DNA compaction and cell vitality: development of an improved methodology
description
article
@en
im Januar 2011 veröffentlichter wissenschaftlicher Artikel
@de
wetenschappelijk artikel
@nl
наукова стаття, опублікована в січні 2011
@uk
name
The TUNEL assay consistently u ...... ent of an improved methodology
@en
The TUNEL assay consistently u ...... ent of an improved methodology
@nl
type
label
The TUNEL assay consistently u ...... ent of an improved methodology
@en
The TUNEL assay consistently u ...... ent of an improved methodology
@nl
prefLabel
The TUNEL assay consistently u ...... ent of an improved methodology
@en
The TUNEL assay consistently u ...... ent of an improved methodology
@nl
P2860
P1476
The TUNEL assay consistently u ...... ent of an improved methodology
@en
P2093
L A Mitchell
P2860
P356
10.1111/J.1365-2605.2009.01042.X
P577
2011-02-01T00:00:00Z