One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome - Test2 - elhamkhani - TriplyDB

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

http://www.wikidata.org/entity/Q22066288

about

Development of an artificial cell, from self-organization to computation and self-reproduction Directed evolution combined with synthetic biology strategies expedite semi-rational engineering of genes and genomes Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones Design and synthesis of a minimal bacterial genome Creation of a bacterial cell controlled by a chemically synthesized genome Rational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single Reaction Design and development of synthetic microbial platform cells for bioenergy Engineering microbial factories for synthesis of value-added products Programming cells: towards an automated 'Genetic Compiler'Protein engineering in designing tailored enzymes and microorganisms for biofuels production How to make a minimal genome for synthetic minimal cell Versatile genetic assembly system (VEGAS) to assemble pathways for expression in S. cerevisiae Pathway swapping: Toward modular engineering of essential cellular processes.Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches Synthetic biology approaches in drug discovery and pharmaceutical biotechnology.Efficient size-independent chromosome delivery from yeast to cultured cell lines Total synthesis of a functional designer eukaryotic chromosome.Direct transfer of whole genomes from bacteria to yeast.PCRless library mutagenesis via oligonucleotide recombination in yeast Yeast oligo-mediated genome engineering (YOGE).Single-step linker-based combinatorial assembly of promoter and gene cassettes for pathway engineering.Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems?Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries.Cloning whole bacterial genomes in yeast.Using DNA microarrays to assay part function RMDAP: a versatile, ready-to-use toolbox for multigene genetic transformation Reiterative Recombination for the in vivo assembly of libraries of multigene pathways.Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases Update on designing and building minimal cells.Synthetic genomes: The next step for the synthetic genome.PGASO: A synthetic biology tool for engineering a cellulolytic yeast.A set of dual promoter vectors for high throughput cloning, screening, and protein expression in eukaryotic and prokaryotic systems from a single plasmid.Large-scale de novo DNA synthesis: technologies and applications.Reassembly of functionally intact environmental DNA-derived biosynthetic gene clusters Recent advances in DNA assembly technologies Synthetic biology: putting synthesis into biology.Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay.Direct cloning of isogenic murine DNA in yeast and relevance of isogenicity for targeting in embryonic stem cells.

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P2860

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

http://www.wikidata.org/entity/Q22066288

description

2008 nî lūn-bûn

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2008 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած

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2008 թվականի դեկտեմբերին հրատարակված գիտական հոդված

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PNAS.0811011106

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Proceedings of the National Academy of Sciences of the United States of America

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One-step assembly in yeast of ...... c Mycoplasma genitalium genome

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Daniel G Gibson

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Gwynedd A Benders

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Jayshree Zaveri

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Kevin C Axelrod

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Michael G Montague

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Mikkel A Algire

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Monzia Moodie

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P2860

Transformation of yeast

Rapid generation of long synthetic tandem repeats and its application for analysis in human artificial chromosome formation.

Yeast transformation: a model system for the study of recombination

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome

Linker-mediated recombinational subcloning of large DNA fragments using yeast

Mapping by multifragment cloning in vivo

Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome.

Nonhomologous end joining in yeast.

Eukaryotic replicators and associated protein complexes.

Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine.

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20404-9

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2284269

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10.1073/PNAS.0811011106

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51

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105

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J. Craig Venter

Clyde A Hutchison

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2008-12-23T00:00:00Z

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23654837

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19073939

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Mycoplasma genitalium

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2600582

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