High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering - Test2 - elhamkhani - TriplyDB

High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering

High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering

http://www.wikidata.org/entity/Q24658210

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Protein stability: computation, sequence statistics, and new experimental methods Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization Biochemical Characterization and Crystal Structures of a Fungal Family 3 -Glucosidase, Cel3A from Hypocrea jecorina Cysteine-free rop: A four-helix bundle core mutant has wild-type stability and structure but dramatically different unfolding kinetics A conserved spider silk domain acts as a molecular switch that controls fibre assembly Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation Clostridium perfringensepsilon toxin H149A mutant as a platform for receptor binding studies Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin Effects of protein engineering and rational mutagenesis on crystal lattice of single chain antibody fragments Structure of arylamine N-acetyltransferase from Mycobacterium tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity Structural basis for double cofactor specificity in a new formate dehydrogenase from the acidobacterium Granulicella mallensis MP5ACTX8 On the mechanism of action of SJ-172550 in inhibiting the interaction of MDM4 and p53 Structural and biochemical characterization reveals LysGH15 as an unprecedented "EF-hand-like" calcium-binding phage lysin Thermodynamic instability of viral proteins is a pathogen-associated molecular pattern targeted by human defensins Bipartite Topology of Treponema pallidum Repeat Proteins C/D and I: OUTER MEMBRANE INSERTION, TRIMERIZATION, AND PORIN FUNCTION REQUIRE A C-TERMINAL β-BARREL DOMAIN Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2.Pharmacological chaperone for α-crystallin partially restores transparency in cataract models Signatures of protein thermal denaturation and local hydrophobicity in domain specific hydration behavior: a comparative molecular dynamics study.Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia An Ancestral Tryptophanyl-tRNA Synthetase Precursor Achieves High Catalytic Rate Enhancement without Ordered Ground-State Tertiary Structures.Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded Conversion of scFv peptide-binding specificity for crystal chaperone development.Rescue of glaucoma-causing mutant myocilin thermal stability by chemical chaperones.Stabilizing proteins from sequence statistics: the interplay of conservation and correlation in triosephosphate isomerase stability.Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins.The helicase and ATPase activities of RECQL4 are compromised by mutations reported in three human patients Rapid optimization and prototyping for therapeutic antibody-like molecules.Probing the kinetic stabilities of Friedreich's ataxia clinical variants using a solid phase GroEL chaperonin capture platform.Ligands for glaucoma-associated myocilin discovered by a generic binding assay Oxidative stress influences positive strand RNA virus genome synthesis and capping.Resolving hot spots in the C-terminal dimerization domain that determine the stability of the molecular chaperone Hsp90 Scalable high throughput selection from phage-displayed synthetic antibody libraries.Subunit asymmetry and roles of conformational switching in the hexameric AAA+ ring of ClpX pH dependence of the stress regulator DksA Increased Fab thermoresistance via VH-targeted directed evolution.Structure and Sialyllactose Binding of the Carboxy-Terminal Head Domain of the Fibre from a Siadenovirus, Turkey Adenovirus 3.Structural integrity of the ribonuclease H domain in HIV-1 reverse transcriptase.Mechanistic Analysis of the Effect of Deamidation on the Immunogenicity of Anthrax Protective Antigen.The dye SYPRO orange binds to amylin amyloid fibrils but not pre-fibrillar intermediates.Identification of an unfolding intermediate for a DNA lesion bypass polymerase.

P2860

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P2860

High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering

http://www.wikidata.org/entity/Q24658210

description

2009 nî lūn-bûn

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2009 թուականի Մարտին հրատարակուած գիտական յօդուած

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2009 թվականի մարտին հրատարակված գիտական հոդված

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2009年の論文

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2009年論文

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2009年論文

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2009年論文

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2009年論文

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name

High-throughput thermal scanni ...... screen for protein engineering

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High-throughput thermal scanni ...... screen for protein engineering

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High-throughput thermal scanni ...... screen for protein engineering

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type

label

High-throughput thermal scanni ...... screen for protein engineering

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High-throughput thermal scanni ...... screen for protein engineering

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High-throughput thermal scanni ...... screen for protein engineering

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prefLabel

High-throughput thermal scanni ...... screen for protein engineering

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High-throughput thermal scanni ...... screen for protein engineering

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High-throughput thermal scanni ...... screen for protein engineering

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P1433

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P1433

Journal of the American Chemical Society

P1476

High-throughput thermal scanni ...... screen for protein engineering

@en

P2093

Brandon J Sullivan

http://www.w3.org/2001/XMLSchema#string

Jason J Lavinder

http://www.w3.org/2001/XMLSchema#string

P2860

Protein stability promotes evolvability

Protein crystallization by surface entropy reduction: optimization of the SER strategy

The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

Thermofluor-based high-throughput stability optimization of proteins for structural studies

High-density miniaturized thermal shift assays as a general strategy for drug discovery

High-precision, high-throughput stability determinations facilitated by robotics and a semiautomated titrating fluorometer.

Rapid and simple protein-stability screens: application to membrane proteins.

Combinatorial approaches to protein stability and structure.

Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery.

Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination.

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3794-3795

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scholarly article

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3765596

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10.1021/JA8049063

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11

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131

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Thomas J Magliery

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2009-03-01T00:00:00Z

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24206666

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19292479

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