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Cultivation strategies to enhance productivity of Pichia pastoris: A review

Cultivation strategies to enhance productivity of Pichia pastoris: A review

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Novel homologous lactate transporter improves L-lactic acid production from glycerol in recombinant strains of Pichia pastoris Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement Autolysis of Pichia pastoris induced by cold.The effect of hypoxia on the lipidome of recombinant Pichia pastoris.Energy Charge as an Indicator of Pexophagy in Pichia pastoris.Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures.Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates.Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source.Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules.Overexpressing target helper genes enhances secretion and glycosylation of recombinant proteins in Pichia pastoris under simulated microgravity.Systematic evaluation of characteristics of the membrane-based fed-batch shake flask.A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing.Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter.A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production.Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris.Dual-substrate inhibition kinetic studies for recombinant human interferon gamma producing Pichia pastoris.Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris.Demonstration-Scale High-Cell-Density Fermentation of Pichia pastoris.Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris.Molecular Cloning and Characterizations of Xylanase Inhibitor Protein from Wheat (Triticum Aestivum).Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris.The vitamin-sensitive promoter PTHI11 enables pre-defined autonomous induction of recombinant protein production in Pichia pastoris.Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis.Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production.13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids.Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris.Improved microscale cultivation of Pichia pastoris for clonal screening.Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.Comparison of Yeasts as Hosts for Recombinant Protein Production.CO to succinic acid - Estimating the potential of biocatalytic routes An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

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Cultivation strategies to enhance productivity of Pichia pastoris: A review

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Cultivation strategies to enhance productivity of Pichia pastoris: A review

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Cultivation strategies to enhance productivity of Pichia pastoris: A review

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Cultivation strategies to enhance productivity of Pichia pastoris: A review

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Cultivation strategies to enhance productivity of Pichia pastoris: A review

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