Simple knockout by electroporation of engineered endonucleases into intact rat embryos
about
Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-newSimple Genome Editing of Rodent Intact Embryos by ElectroporationGenetic manipulation of reptilian embryos: toward an understanding of cortical development and evolutionEasi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.Optically-controlled platforms for transfection and single- and sub-cellular surgery.Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing.Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing.GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in miceEfficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos.Rat Breeding Parameters According to Floor Space Available in Cage.Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model CreationAsymmetric Waveforms Decrease Lethal Thresholds in High Frequency Irreversible Electroporation Therapies.Advances in transgenic animal models and techniques.Toward establishing model organisms for marine protists: Successful transfection protocols for Parabodo caudatus (Kinetoplastida: Excavata).In vivo and in vitro disease modeling with CRISPR/Cas9.Efficient gene-targeting in rat embryonic stem cells by CRISPR/Cas and generation of human kynurenine aminotransferase II (KAT II) knock-in rat.Efficient generation of conditional knockout mice via sequential introduction of lox sites.Crotamine, a cell-penetrating peptide, is able to translocate parthenogenetic and in vitro fertilized bovine embryos but does not improve exogenous DNA expression.Non-electrolytic microelectroporation.Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation.Genome Editing of Rat.Genome Editing in Mouse and Rat by Electroporation.Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing.Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.En masse lentiviral gene delivery to mouse fertilized eggs via laser perforation of zona pellucida.i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.Successful production of genome-edited rats by the rGONAD method.Reproductive technologies for the generation and maintenance of valuable animal strains.An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.CLICK: one-step generation of conditional knockout mice.i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats
P2860
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P2860
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
description
2014 nî lūn-bûn
@nan
2014 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2014 թվականի հոտեմբերին հրատարակված գիտական հոդված
@hy
2014年の論文
@ja
2014年論文
@yue
2014年論文
@zh-hant
2014年論文
@zh-hk
2014年論文
@zh-mo
2014年論文
@zh-tw
2014年论文
@wuu
name
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@ast
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@en
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@nl
type
label
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@ast
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@en
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@nl
prefLabel
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@ast
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@en
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@nl
P2860
P356
P1433
P1476
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
@en
P2093
Takehito Kaneko
Tomoji Mashimo
P2860
P2888
P356
10.1038/SREP06382
P407
P577
2014-10-01T00:00:00Z
P5875
P6179
1021972084