Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases.
about
A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches.Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases.Probing the limits of genetic recoding in essential genes.The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populationsRepetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker.Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli.A simple and effective method for construction of Escherichia coli strains proficient for genome engineering.Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA.Differential requirements of singleplex and multiplex recombineering of large DNA constructs.Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering.Accelerated genome engineering through multiplexing.Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli.Combinatorial approaches for inverse metabolic engineering applications.Rational optimization of tolC as a powerful dual selectable marker for genome engineering.Recombineering to homogeneity: extension of multiplex recombineering to large-scale genome editing.Precision genome engineering in lactic acid bacteria.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Strategies for cloning and manipulating natural and synthetic chromosomes.Novel Technologies for Optimal Strain Breeding.The Ssr protein (T1E_1405) from Pseudomonas putida DOT-T1E enables oligonucleotide-based recombineering in platform strain P. putida EM42.Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress.A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities.Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection.Bacterial DNA polymerases participate in oligonucleotide recombination.Potent O-antigen-deficient (rough) mutants of Salmonella Typhimurium secreting Lawsonia intracellularis antigens enhance immunogenicity and provide single-immunization protection against proliferative enteropathy and salmonellosis in a murine model.
P2860
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P2860
Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases.
description
2012 nî lūn-bûn
@nan
2012 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
2012 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
2012年の論文
@ja
2012年論文
@yue
2012年論文
@zh-hant
2012年論文
@zh-hk
2012年論文
@zh-mo
2012年論文
@zh-tw
2012年论文
@wuu
name
Improving lambda red genome en ...... moval of endogenous nucleases.
@ast
Improving lambda red genome en ...... moval of endogenous nucleases.
@en
Improving lambda red genome en ...... moval of endogenous nucleases.
@nl
type
label
Improving lambda red genome en ...... moval of endogenous nucleases.
@ast
Improving lambda red genome en ...... moval of endogenous nucleases.
@en
Improving lambda red genome en ...... moval of endogenous nucleases.
@nl
prefLabel
Improving lambda red genome en ...... moval of endogenous nucleases.
@ast
Improving lambda red genome en ...... moval of endogenous nucleases.
@en
Improving lambda red genome en ...... moval of endogenous nucleases.
@nl
P2093
P2860
P1433
P1476
Improving lambda red genome en ...... moval of endogenous nucleases.
@en
P2093
Christopher J Gregg
Harris H Wang
Joshua A Mosberg
Marc J Lajoie
P2860
P304
P356
10.1371/JOURNAL.PONE.0044638
P407
P577
2012-09-05T00:00:00Z