Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor.
about
Overexpression of human cardiac troponin-I and troponin-C in Escherichia coli and their purification and characterisation. Two point mutations allow high-level expression of troponin-IMutants of Tn3 resolvase which do not require accessory binding sites for recombination activityCooperative DNA binding of the human HoxB5 (Hox-2.1) protein is under redox regulation in vitroProtein production and purificationProduction of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expressionFunctional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae.Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesiclesIdentification of RamA, a novel LuxR-type transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum.RamB, a novel transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicumInsertion of foreign T cell epitopes in human tumor necrosis factor alpha with minimal effect on protein structure and biological activity.Identification and molecular analysis of PcsB, a protein required for cell wall separation of group B streptococcusDisplay of functional beta-lactamase inhibitory protein on the surface of M13 bacteriophage.Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylaseBacterial SOS checkpoint protein SulA inhibits polymerization of purified FtsZ cell division protein.Two functionally distinct regions upstream of the cbbI operon of Rhodobacter sphaeroides regulate gene expression.Expression, isolation, and purification of soluble and insoluble biotinylated proteins for nerve tissue regeneration.IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins.Computational design of synthetic regulatory networks from a genetic library to characterize the designability of dynamical behaviorsStringent chemical and thermal regulation of recombinant gene expression by vaccinia virus vectors in mammalian cells.The novel fibrinogen-binding protein FbsB promotes Streptococcus agalactiae invasion into epithelial cells.Engineering of a synthetic electron conduit in living cells.Autogenous translational regulation of the ribosomal MvaL1 operon in the archaebacterium Methanococcus vannielii.An improved method and cost effective strategy for soluble expression and purification of human N-myristoyltransferase 1 in E. coli.Recombinant protein expression in Escherichia coli: advances and challengesBoundaries of the origin of replication: creation of a pET-28a-derived vector with p15A copy control allowing compatible coexistence with pET vectors.Common and variable contributions of Fis residues to high-affinity binding at different DNA sequencesMicrocin e492 amyloid formation is retarded by posttranslational modification.A self-initiating eukaryotic transient gene expression system based on contransfection of bacteriophage T7 RNA polymerase and DNA vectors containing a T7 autogeneSynthesis of catalytically active form III ribulose 1,5-bisphosphate carboxylase/oxygenase in archaea.High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operatorMulti-input regulation and logic with T7 promoters in cells and cell-free systems.The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli.Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coliSynthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes.Auto-induction expression of human consensus interferon-alpha in Escherichia coliCharacterization of elongating T7 and SP6 RNA polymerases and their response to a roadblock generated by a site-specific DNA binding protein.Expression optimization and synthetic gene networks in cell-free systems.Escherichia coli produces a cytoplasmic alpha-amylase, AmyARegulatory circuit based on autogenous activation-repression: roles of C-boxes and spacer sequences in control of the PvuII restriction-modification system.
P2860
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P2860
Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor.
description
1991 nî lūn-bûn
@nan
1991 թուականի Մայիսին հրատարակուած գիտական յօդուած
@hyw
1991 թվականի մայիսին հրատարակված գիտական հոդված
@hy
1991年の論文
@ja
1991年論文
@yue
1991年論文
@zh-hant
1991年論文
@zh-hk
1991年論文
@zh-mo
1991年論文
@zh-tw
1991年论文
@wuu
name
Controlling basal expression i ...... 7 promoter with lac repressor.
@ast
Controlling basal expression i ...... 7 promoter with lac repressor.
@en
Controlling basal expression i ...... 7 promoter with lac repressor.
@nl
type
label
Controlling basal expression i ...... 7 promoter with lac repressor.
@ast
Controlling basal expression i ...... 7 promoter with lac repressor.
@en
Controlling basal expression i ...... 7 promoter with lac repressor.
@nl
prefLabel
Controlling basal expression i ...... 7 promoter with lac repressor.
@ast
Controlling basal expression i ...... 7 promoter with lac repressor.
@en
Controlling basal expression i ...... 7 promoter with lac repressor.
@nl
P1476
Controlling basal expression i ...... 7 promoter with lac repressor.
@en
P2093
Dubendorff JW
Studier FW
P356
10.1016/0022-2836(91)90856-2
P407
P577
1991-05-01T00:00:00Z