An overview of enzymatic reagents for the removal of affinity tags.
about
A tobacco etch virus protease with increased substrate tolerance at the P1' positionEngineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial librariesFunctional cross-talk between distant domains of chikungunya virus non-structural protein 2 is decisive for its RNA-modulating activityLarge-scale production and protein engineering of G protein-coupled receptors for structural studiesTo fuse or not to fuse: what is your purpose?Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 systemHigh-throughput recombinant protein expression in Escherichia coli: current status and future perspectivesRecombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studiesAtomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor.A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removalSynthesis and characterization of different immunogenic viral nanoconstructs from rotavirus VP6 inner capsid protein.His-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant: Gain or Loss?Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteasesRecombinant protein expression in Escherichia coli: advances and challengesProduction of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli.Site-specific protein labeling via sortase-mediated transpeptidation.Prokaryotic soluble overexpression and purification of bioactive human growth hormone by fusion to thioredoxin, maltose binding protein, and protein disulfide isomerase.Histones: at the crossroads of peptide and protein chemistry.Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant.Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3).Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography.Milk-deteriorating exoenzymes from Pseudomonas fluorescens 041 isolated from refrigerated raw milkBiological activities of histidine-rich peptides; merging biotechnology and nanomedicine.Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein ProductionNew ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag.Evolutionary Selection on Barrier Activity: Bar1 Is an Aspartyl Protease with Novel Substrate Specificity.Structure-Function Analysis of a Mixed-linkage β-Glucanase/Xyloglucanase from the Key Ruminal Bacteroidetes Prevotella bryantii B(1)4.IMAC capture of recombinant protein from unclarified mammalian cell feed streamsGlobal characterization of in vivo enzyme catalytic rates and their correspondence to in vitro kcat measurementsMolecular mechanism of activation-triggered subunit exchange in Ca(2+)/calmodulin-dependent protein kinase IIA dual protease approach for expression and affinity purification of recombinant proteinsSeveral affinity tags commonly used in chromatographic purification.Post-translational control of genetic circuits using Potyvirus proteases.Inteins: Nature's Gift to Protein Chemists.A new fusion protein platform for quantitatively measuring activity of multiple proteases.Production of prone-to-aggregate proteins.Research applications of proteolytic enzymes in molecular biology.A Chimeric Affinity Tag for Efficient Expression and Chromatographic Purification of Heterologous Proteins from Plants.
P2860
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P2860
An overview of enzymatic reagents for the removal of affinity tags.
description
2011 nî lūn-bûn
@nan
2011 թուականի Օգոստոսին հրատարակուած գիտական յօդուած
@hyw
2011 թվականի օգոստոսին հրատարակված գիտական հոդված
@hy
2011年の論文
@ja
2011年論文
@yue
2011年論文
@zh-hant
2011年論文
@zh-hk
2011年論文
@zh-mo
2011年論文
@zh-tw
2011年论文
@wuu
name
An overview of enzymatic reagents for the removal of affinity tags.
@ast
An overview of enzymatic reagents for the removal of affinity tags.
@en
type
label
An overview of enzymatic reagents for the removal of affinity tags.
@ast
An overview of enzymatic reagents for the removal of affinity tags.
@en
prefLabel
An overview of enzymatic reagents for the removal of affinity tags.
@ast
An overview of enzymatic reagents for the removal of affinity tags.
@en
P2860
P1476
An overview of enzymatic reagents for the removal of affinity tags.
@en
P2093
David S Waugh
P2860
P304
P356
10.1016/J.PEP.2011.08.005
P577
2011-08-19T00:00:00Z