RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
about
Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesisNon-Standard Genetic Codes Define New Concepts for Protein EngineeringEngine out of the chassis: cell-free protein synthesis and its usesGenetic code expansion as a tool to study regulatory processes of transcriptionCell-free biology: exploiting the interface between synthetic biology and synthetic chemistryRecent developments of engineered translational machineries for the incorporation of non-canonical amino acids into polypeptidesMultiply labeling proteins for studies of folding and stabilityExpanded cellular amino acid pools containing phosphoserine, phosphothreonine, and phosphotyrosineTowards reassigning the rare AGG codon in Escherichia coliRevealing the amino acid composition of proteins within an expanded genetic codeGenomically recoded organisms expand biological functionsSense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansionRepurposing the translation apparatus for synthetic biologyHigh-Yield, Zero-Leakage Expression System with a Translational Switch Using Site-Specific Unnatural Amino Acid IncorporationSite-specific bioconjugation of a murine dihydrofolate reductase enzyme by copper(I)-catalyzed azide-alkyne cycloaddition with retained activityAt the Interface of Chemical and Biological Synthesis: An Expanded Genetic Code.Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.Site-specific fatty acid-conjugation to prolong protein half-life in vivoRF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates.Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation.Distinct roles for release factor 1 and release factor 2 in translational quality control.Genomes by design.Response and adaptation of Escherichia coli to suppression of the amber stop codonA genetic replacement system for selection-based engineering of essential proteinsInvestigating the role of a backbone to substrate hydrogen bond in OMP decarboxylase using a site-specific amide to ester substitution.Genetically encoded libraries of nonstandard peptides.Genetic PEGylationProtein conjugation with genetically encoded unnatural amino acids.Histones: at the crossroads of peptide and protein chemistry.Methods to Make Homogenous Antibody Drug Conjugates.Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1Inteins as traceless purification tags for unnatural amino acid proteins.Expanding the amino acid repertoire of ribosomal polypeptide synthesis via the artificial division of codon boxes.Re-exploration of the Codon Context Effect on Amber Codon-Guided Incorporation of Noncanonical Amino Acids in Escherichia coli by the Blue-White Screening Assay.Release factor one is nonessential in Escherichia coli.Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coliGlobal analysis of translation termination in E. coli.Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion.Rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acidsCRISPRi-Manipulation of Genetic Code Expansion via RF1 for Reassignment of Amber Codon in Bacteria.
P2860
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P2860
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
description
2011 nî lūn-bûn
@nan
2011 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
2011 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
2011年の論文
@ja
2011年論文
@yue
2011年論文
@zh-hant
2011年論文
@zh-hk
2011年論文
@zh-mo
2011年論文
@zh-tw
2011年论文
@wuu
name
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@ast
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@en
type
label
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@ast
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@en
prefLabel
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@ast
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@en
P2093
P2860
P356
P1476
RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.
@en
P2093
David B F Johnson
Jeffrey K Takimoto
Jianfeng Xu
Joseph R Ecker
Matthew D Schultz
Robert J Schmitz
Steven P Briggs
Zheng Xiang
Zhouxin Shen
P2860
P2888
P304
P356
10.1038/NCHEMBIO.657
P577
2011-09-18T00:00:00Z
P5875
P6179
1043358251