Single-molecule imaging of a fluorescent unnatural amino acid incorporated into nicotinic receptors
about
Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacologyIn vivo incorporation of non-canonical amino acids by using the chemical aminoacylation strategy: a broadly applicable mechanistic toolFluorescence applications in molecular neurobiologyNicotinic acetylcholine receptor alpha7 subunits with a C2 cytoplasmic loop yellow fluorescent protein insertion form functional receptors.Nano-assembly of amyloid β peptide: role of the hairpin fold.Efficient synthesis and in vivo incorporation of acridon-2-ylalanine, a fluorescent amino acid for lifetime and Förster resonance energy transfer/luminescence resonance energy transfer studies.Evaluation of fluorophores to label SNAP-tag fused proteins for multicolor single-molecule tracking microscopy in live cells.Counting bungarotoxin binding sites of nicotinic acetylcholine receptors in mammalian cells with high signal/noise ratios.Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures.Förster resonance energy transfer (FRET) correlates of altered subunit stoichiometry in cys-loop receptors, exemplified by nicotinic α4β2.Stoichiometry of the human glycine receptor revealed by direct subunit counting.Dynamics of internal pore opening in K(V) channels probed by a fluorescent unnatural amino acid.Nicotine is a selective pharmacological chaperone of acetylcholine receptor number and stoichiometry. Implications for drug discovery.Probing the role of backbone hydrogen bonding in a critical beta sheet of the extracellular domain of a cys-loop receptor.The structural basis of function in Cys-loop receptors.FRET microscopy in the living cell: different approaches, strengths and weaknesses.Deciphering the subunit composition of multimeric proteins by counting photobleaching steps.Patch-clamp fluorometry: electrophysiology meets fluorescence.Incorporation of Non-Canonical Amino Acids.Cellular encoding of Cy dyes for single-molecule imaging.Correlated optical and electrical single-molecule measurements reveal conformational diffusion from ligand binding to channel gating in the nicotinic acetylcholine receptor.Conformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid.Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells.Visualizing the growth and dynamics of liquid-ordered domains during lipid bilayer folding in a microfluidic chip.
P2860
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P2860
Single-molecule imaging of a fluorescent unnatural amino acid incorporated into nicotinic receptors
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on January 2009
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vedecký článok
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vetenskaplig artikel
@sv
videnskabelig artikel
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vědecký článek
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name
Single-molecule imaging of a f ...... rated into nicotinic receptors
@en
Single-molecule imaging of a f ...... ated into nicotinic receptors.
@nl
type
label
Single-molecule imaging of a f ...... rated into nicotinic receptors
@en
Single-molecule imaging of a f ...... ated into nicotinic receptors.
@nl
prefLabel
Single-molecule imaging of a f ...... rated into nicotinic receptors
@en
Single-molecule imaging of a f ...... ated into nicotinic receptors.
@nl
P2093
P2860
P1433
P1476
Single-molecule imaging of a f ...... rated into nicotinic receptors
@en
P2093
Dennis A Dougherty
Mohammed I Dibas
Rigo Pantoja
P2860
P304
P356
10.1016/J.BPJ.2008.09.034
P407
P577
2009-01-01T00:00:00Z