Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomesRegional and temporal specialization in the nucleus: a transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle.Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division.Genomics meets nanoscience: probing genes and the cell nucleus at 10-9 metersFrom linear genome sequence to three-dimensional organization of the cell nucleusPhysical mechanisms behind the large scale features of chromatin organizationCorrelative fluorescence and electron microscopy on ultrathin cryosections: bridging the resolution gap.Measuring the size of biological nanostructures with spatially modulated illumination microscopyIntermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associationsPoised transcription factories prime silent uPA gene prior to activation.CryoFISH: fluorescence in situ hybridization on ultrathin cryosections.Gene positioning.Polycomb targets seek closest neighbours.Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2.Specialized transcription factories within mammalian nuclei.Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitmentProteomic analysis of mitotic RNA polymerase II reveals novel interactors and association with proteins dysfunctional in disease.Models of chromosome structure.Three-dimensional genome architecture: players and mechanisms.Mean Expression of the X-Chromosome is Associated with Neuronal DensitySplicing speckles are not reservoirs of RNA polymerase II, but contain an inactive form, phosphorylated on serine2 residues of the C-terminal domain.Cellular genomics: which genes are transcribed, when and where?Genome function and nuclear architecture: from gene expression to nanoscience.Transcription factories: quantitative studies of nanostructures in the mammalian nucleus.Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells.Complexity of chromatin folding is captured by the strings and binders switch model.Transcription and chromatin organization of a housekeeping gene cluster containing an integrated beta-globin locus control region.Methylation of RNA polymerase II non-consensus Lysine residues marks early transcription in mammalian cells.Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators.Chromosome organization: new facts, new models.The localization of sites containing nascent RNA and splicing factors.Advances in imaging the interphase nucleus using thin cryosections.Polycomb associates genome-wide with a specific RNA polymerase II variant, and regulates metabolic genes in ESCs.Polymer models of chromatin organizationFunctional organisation of the genome during interphase.A polymer model explains the complexity of large-scale chromatin folding.Eukaryotic gene regulation in three dimensions and its impact on genome evolution.Mechanisms regulating S phase progression in mammalian cells.The molecular basis for stability of heterochromatin-mediated silencing in mammalsModifications of RNA polymerase II are pivotal in regulating gene expression states.
P50
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P50
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Ana Pombo
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Ana Pombo
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Ana Pombo
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Ana Pombo
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Ana Pombo
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Ana Pombo
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Ana Pombo
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