An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.
about
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studiesInsight into the mode of action of the LRRK2 Y1699C pathogenic mutantTransient and stable knockdown of the integrase cofactor LEDGF/p75 reveals its role in the replication cycle of human immunodeficiency virusDeveloping a programmed restriction endonuclease for highly specific DNA cleavageTo get what we aim for--progress in diversity generation methodsStructural basis for LEAFY floral switch function and similarity with helix-turn-helix proteinsThe C-Terminal Domain of the MutL Homolog from Neisseria gonorrhoeae Forms an Inverted HomodimerCrystal structure of a membrane-embedded H+-translocating pyrophosphataseTargeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitorsStrategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expressionEXOG, a novel paralog of Endonuclease G in higher eukaryotesCooperation between engulfment receptors: the case of ABCA1 and MEGF10Functional analysis of an acid adaptive DNA adenine methyltransferase from Helicobacter pylori 26695A symmetric region of the HIV-1 integrase dimerization interface is essential for viral replicationFunctional characterization of UvrD helicases from Haemophilus influenzae and Helicobacter pyloriOmniChange: the sequence independent method for simultaneous site-saturation of five codonsStructural mapping of the coiled-coil domain of a bacterial condensin and comparative analyses across all domains of life suggest conserved features of SMC proteins.PKCalpha: a versatile key for decoding the cellular calcium toolkit.Chemical trapping of the dynamic MutS-MutL complex formed in DNA mismatch repair in Escherichia coli.Mutagenic analysis of functional residues in putative substrate-binding site and acidic domains of vacuolar H+-pyrophosphatase.A novel strategy for the identification of protein-DNA contacts by photocrosslinking and mass spectrometry.Specificity changes in the evolution of type II restriction endonucleases: a biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences.Identifying an interaction site between MutH and the C-terminal domain of MutL by crosslinking, affinity purification, chemical coding and mass spectrometry.Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV.High levels of MeCP2 depress MHC class I expression in neuronal cells.A variant of LEAFY reveals its capacity to stimulate meristem development by inducing RAX1.A Conserved Role for the NAM/miR164 Developmental Module Reveals a Common Mechanism Underlying Carpel Margin Fusion in Monocarpous and Syncarpous Eurosids.Immunohistochemical detection of transgene expression in the brain using small epitope tags.High-affinity insulin binding: insulin interacts with two receptor ligand binding sites.Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production.Distance variations between active sites of H(+)-pyrophosphatase determined by fluorescence resonance energy transfer.Differences in the large extracellular loop between the K(+)-Cl(-) cotransporters KCC2 and KCC4.Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systemsThe HIV-1 integrase mutant R263A/K264A is 2-fold defective for TRN-SR2 binding and viral nuclear import.Membrane topology of the H+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis.Structural basis for stable DNA complex formation by the caspase-activated DNase.An improved dual-tube megaprimer approach for multi-site saturation mutagenesis.SOMA: a single oligonucleotide mutagenesis and cloning approachProperties of arsenite efflux permeases (Acr3) from Alkaliphilus metalliredigens and Corynebacterium glutamicum.Expression of an Mg2+-dependent HIV-1 RNase H construct for drug screening
P2860
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P2860
An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.
description
1998 nî lūn-bûn
@nan
1998年の論文
@ja
1998年論文
@yue
1998年論文
@zh-hant
1998年論文
@zh-hk
1998年論文
@zh-mo
1998年論文
@zh-tw
1998年论文
@wuu
1998年论文
@zh
1998年论文
@zh-cn
name
An improved PCR-mutagenesis st ...... wapping between related genes.
@en
An improved PCR-mutagenesis st ...... wapping between related genes.
@nl
type
label
An improved PCR-mutagenesis st ...... wapping between related genes.
@en
An improved PCR-mutagenesis st ...... wapping between related genes.
@nl
prefLabel
An improved PCR-mutagenesis st ...... wapping between related genes.
@en
An improved PCR-mutagenesis st ...... wapping between related genes.
@nl
P2860
P356
P1476
An improved PCR-mutagenesis st ...... wapping between related genes.
@en
P2093
P2860
P304
P356
10.1093/NAR/26.7.1848
P407
P577
1998-04-01T00:00:00Z