Synthesis of recombinant human procollagen II in a stably transfected tumour cell line (HT1080).
about
Crystal structure and mapping by site-directed mutagenesis of the collagen-binding epitope of an activated form of BM-40/SPARC/osteonectinThe C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1Development and characterization of a eukaryotic expression system for human type II procollagenCollagen fibril formationReducing the effects of intracellular accumulation of thermolabile collagen II mutants by increasing their thermostability in cell culture conditions.Natural and Genetically Engineered Proteins for Tissue Engineering.Targeted insertions of two exogenous collagen genes into both alleles of their endogenous loci in cultured human cells: the insertions are directed by relatively short fragments containing the promoters and the 5' ends of the genesA Streptococcus pyogenes derived collagen-like protein as a non-cytotoxic and non-immunogenic cross-linkable biomaterialTriple helical collagen-like peptides: engineering and applications in matrix biology.Prospects and limitations of the rational engineering of fibrillar collagensMapping of SPARC/BM-40/osteonectin-binding sites on fibrillar collagens.Collagens as biomaterials.R992C (p.R1192C) Substitution in collagen II alters the structure of mutant molecules and induces the unfolded protein response.Bioengineered Collagens.Characterization of human type X procollagen and its NC-1 domain expressed as recombinant proteins in HEK293 cells.Human recombinant alpha1(V) collagen chain. Homotrimeric assembly and subsequent processing.Assembly in vitro of thin and thick fibrils of collagen II from recombinant procollagen II. The monomers in the tips of thick fibrils have the opposite orientation from monomers in the growing tips of collagen I fibrils.Biosynthesis of recombinant human pro-alpha 1(III) chains in a baculovirus expression system: production of disulphide-bonded and non-disulphide-bonded species containing full-length triple helices.Production of recombinant human type I procollagen trimers using a four-gene expression system in the yeast Saccharomyces cerevisiae.Characterization of human type III collagen expressed in a baculovirus system. Production of a protein with a stable triple helix requires coexpression with the two types of recombinant prolyl 4-hydroxylase subunit.Lack of a phenotype in transgenic mice aberrantly expressing COL2A1 mRNA because of highly selective post-transcriptional down-regulationStable transfection of human fetal chondrocytes with a type II procollagen minigene: expression of the mutant protein and alterations in the structure of the extracellular matrix in vitro.Assessment of prokaryotic collagen-like sequences derived from streptococcal Scl1 and Scl2 proteins as a source of recombinant GXY polymers.Mapping critical sites in collagen II for rational design of gene-engineered proteins for cell-supporting materials.Two novel COL1A1 mutations in patients with osteogenesis imperfecta (OI) affect the stability of the collagen type I triple-helix.Evaluation of the immunogenicity and cell compatibility of avian collagen for biomedical applications.Impact of Arginine to Cysteine Mutations in Collagen II on Protein Secretion and Cell Survival.Testing the utility of rationally engineered recombinant collagen-like proteins for applications in tissue engineering.Recombinant procollagen II: Deletion of D period segments identifies sequences that are required for helix stabilization and generates a temperature-sensitive N-proteinase cleavage site.Genetically modified human type II collagen for N- and C-terminal covalent tagging
P2860
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P2860
Synthesis of recombinant human procollagen II in a stably transfected tumour cell line (HT1080).
description
1994 nî lūn-bûn
@nan
1994年の論文
@ja
1994年論文
@yue
1994年論文
@zh-hant
1994年論文
@zh-hk
1994年論文
@zh-mo
1994年論文
@zh-tw
1994年论文
@wuu
1994年论文
@zh
1994年论文
@zh-cn
name
Synthesis of recombinant human ...... ted tumour cell line (HT1080).
@en
type
label
Synthesis of recombinant human ...... ted tumour cell line (HT1080).
@en
prefLabel
Synthesis of recombinant human ...... ted tumour cell line (HT1080).
@en
P2093
P2860
P356
P1433
P1476
Synthesis of recombinant human ...... cted tumour cell line (HT1080)
@en
P2093
P2860
P356
10.1042/BJ2980031
P407
P478
298 ( Pt 1)
P577
1994-02-01T00:00:00Z