West Virginia Route 527 is a north–south state highway located entirely in the Huntington, West Virginia area. The southern terminus of the route is at Interstate 64 exit 8 south of the city, where WV 527 becomes West Virginia Route 152 upon crossing the expressway. The northern terminus is on the West Virginia Department of Transportation-maintained Robert C. Byrd Bridge, better known as the Sixth Street Bridge, where the route becomes State Route 527 upon crossing into Ohio. In Ohio, SR 527 continues northward to State Route 7 in Chesapeake.