Anomalous scattering
In X-ray crystallography, anomalous scattering refers to a change in a diffracting X-ray’s phase that is unique from the rest of the atoms in a crystal due to strong X-ray absorbance. The amount of energy that individual atoms absorb depends on their atomic number. The relatively light atoms found in proteins such as carbon, nitrogen, and oxygen do not contribute to anomalous scattering at normal X-ray wavelengths used for X-ray crystallography. Thus, in order to observe anomalous scattering, a heavy atom must be native to the protein or a heavy atom derivative should be made. In addition, the X-ray’s wavelength should be close to the heavy atom’s absorption edge.
primaryTopic
Anomalous scattering
In X-ray crystallography, anomalous scattering refers to a change in a diffracting X-ray’s phase that is unique from the rest of the atoms in a crystal due to strong X-ray absorbance. The amount of energy that individual atoms absorb depends on their atomic number. The relatively light atoms found in proteins such as carbon, nitrogen, and oxygen do not contribute to anomalous scattering at normal X-ray wavelengths used for X-ray crystallography. Thus, in order to observe anomalous scattering, a heavy atom must be native to the protein or a heavy atom derivative should be made. In addition, the X-ray’s wavelength should be close to the heavy atom’s absorption edge.
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En diffraction de rayons X, la ...... astique résonante de rayons X.
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In X-ray crystallography, anom ...... heavy atom’s absorption edge.
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Wikipage page ID
21,346,659
Wikipage revision ID
545,557,058
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En diffraction de rayons X, la ...... t du coefficient d'absorption.
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In X-ray crystallography, anom ...... heavy atom’s absorption edge.
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Anomalous scattering
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Diffusion anomale
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