Jamestown is a town on the N6 national road 58 km south of Aliwal North and 105 km north of Queenstown in the Joe Gqabi District Municipality of the Eastern Cape, South Africa. It is at the centre of a fertile sheep, cattle and wheat-farming area and was the terminus of a branch railway line from Molteno. It was named after James Wagenaar, original owner of the farm on which the town was laid out. The Jamestown Hospital, which was previously provincially aided, has since January 2011 been fully operated by the Eastern Cape Department of Health.