The 2001 1000 Guineas Stakes was a horse race held at Newmarket Racecourse on Sunday 6 May 2001. It was the 188th running of the 1000 Guineas. The winner was Ahmed Al Maktoum's Ameerat, a British-bred bay filly trained at Newmarket, Suffolk by Michael Jarvis and ridden by Philip Robinson. Ameerat's victory was the first in the race for her owner and trainer and the second for Robinson who had won on Pebbles in 1985.