5-Bromo-4-chloro-3-indolyl phosphate
5-Bromo-4-chloro-3-indolyl phosphate (BCIP, X-phosphate, XP) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It is, for example, used in immunoblotting, in situ hybridization, and immunohistochemistry, often in combination with nitro blue tetrazolium chloride (NBT). 5-bromo-4-chloro-3-indoxyl is oxidized by atmospheric oxygen to form the blue dye 5,5′-dibromo-4,4′-dichloro-indigo. It is also oxidized by nitroblue tetrazolium (NBT), which forms an insoluble dark blue diformazan precipitate after reduction. Alkaline phosphatase is commonly conjugated to secondary antibodies.
Wikipage redirect
primaryTopic
5-Bromo-4-chloro-3-indolyl phosphate
5-Bromo-4-chloro-3-indolyl phosphate (BCIP, X-phosphate, XP) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It is, for example, used in immunoblotting, in situ hybridization, and immunohistochemistry, often in combination with nitro blue tetrazolium chloride (NBT). 5-bromo-4-chloro-3-indoxyl is oxidized by atmospheric oxygen to form the blue dye 5,5′-dibromo-4,4′-dichloro-indigo. It is also oxidized by nitroblue tetrazolium (NBT), which forms an insoluble dark blue diformazan precipitate after reduction. Alkaline phosphatase is commonly conjugated to secondary antibodies.
has abstract
5-Brom-4-chlor-3-indolylphosph ...... m Alkalische Phosphatase (AP).
@de
5-Bromo-4-chloro-3-indolyl pho ...... gated to secondary antibodies.
@en
IUPAC name
(5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate
@en
thumbnail
Wikipage page ID
30,860,353
Wikipage revision ID
674,791,399
ImageAlt
Ball-and-stick model of the BCIP molecule
Skeletal formula of BCIP
ImageFile
othernames
Verifiedfields
verifiedrevid
445,341,734
Watchedfields
subject
hypernym
comment
5-Brom-4-chlor-3-indolylphosph ...... m Alkalische Phosphatase (AP).
@de
5-Bromo-4-chloro-3-indolyl pho ...... gated to secondary antibodies.
@en
label
5-Brom-4-chlor-3-indoxylphosphat
@de
5-Bromo-4-chloro-3-indolyl phosphate
@en
wasDerivedFrom
depiction
isPrimaryTopicOf
name
BCIP
@en