Cloned enzyme donor immunoassay
A cloned enzyme donor immunoassay (CEDIA) is a competitive homogenous enzyme immunoassay. This assay makes use of two component fragments of an enzyme which are each individually inactive. Under the right conditions in solution these fragments can spontaneously reassemble to form the active enzyme. For use in biochemical assays one of the enzyme fragments is attached to an analyte of interest. The analyte-enzyme-fragment-conjugate is still able to reassemble with the other enzyme fragment to form an active enzyme. However it is unable to do this if the analyte is bound to an antibody.
primaryTopic
Cloned enzyme donor immunoassay
A cloned enzyme donor immunoassay (CEDIA) is a competitive homogenous enzyme immunoassay. This assay makes use of two component fragments of an enzyme which are each individually inactive. Under the right conditions in solution these fragments can spontaneously reassemble to form the active enzyme. For use in biochemical assays one of the enzyme fragments is attached to an analyte of interest. The analyte-enzyme-fragment-conjugate is still able to reassemble with the other enzyme fragment to form an active enzyme. However it is unable to do this if the analyte is bound to an antibody.
has abstract
A cloned enzyme donor immunoas ...... therefore low enzyme activity.
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المقايسة المناعية المانحة للإن ...... كون الفعالية الإنزيمية منخفضة.
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Wikipage page ID
38,080,816
Wikipage revision ID
708,772,321
name
Cloned enzyme donor immunoassay
purpose
To determine the quantity of analyte in a sample
type
Competitive homogenous enzyme immunoassay
comment
A cloned enzyme donor immunoas ...... alyte is bound to an antibody.
@en
المقايسة المناعية المانحة للإن ...... الأخرى وتشكيل الانزيم الفعال.
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label
Cloned enzyme donor immunoassay
@en
المقايسة المناعية المانحة للانزيم المنسل
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