Fura-2-acetoxymethyl ester
Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeable derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium conc
Wikipage disambiguates
primaryTopic
Fura-2-acetoxymethyl ester
Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeable derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium conc
has abstract
Fura-2-AM ist ein membrangängi ...... und dient als Ca2+-Indikator.
@de
Fura-2-acetoxymethyl ester, of ...... lcium concentrations in cells.
@en
IUPAC name
2-yl]oxazole-5-carboxylate
@en
Acetoxymethyl 2-[5-[bis[(aceto ...... hyl-phenoxy]ethoxy]benzofuran-
@en
thumbnail
Wikipage page ID
Wikipage revision ID
670,382,948
imagesize
othernames
verifiedrevid
427,818,427
hypernym
comment
Fura-2-AM ist ein membrangängi ...... und dient als Ca2+-Indikator.
@de
Fura-2-acetoxymethyl ester, of ...... tempting to image calcium conc
@en
label
Fura-2-acetoxymethyl ester
@en
Fura-2AM
@de