Intensity fading MALDI mass spectrometry
Intensity-fading MALDI is a term coined to rename an existing method originally reported in 1999 to indirectly study a Protein–protein interaction or other protein complex and the same year applied to a biological mixture to study the antigenicity of the influenza virus. It involves treating a protein and a potential binding partner with a site-specific endoproteinase with the binding sites identified by their reduced area (or intensity) in a MALDI mass spectrum compared to that of non-bound protein control. The basis of the approach and a range of applications including those employing gel based separations, drug-protein interactions and the relative affinity of such interactions, are described in a recent review article.
primaryTopic
Intensity fading MALDI mass spectrometry
Intensity-fading MALDI is a term coined to rename an existing method originally reported in 1999 to indirectly study a Protein–protein interaction or other protein complex and the same year applied to a biological mixture to study the antigenicity of the influenza virus. It involves treating a protein and a potential binding partner with a site-specific endoproteinase with the binding sites identified by their reduced area (or intensity) in a MALDI mass spectrum compared to that of non-bound protein control. The basis of the approach and a range of applications including those employing gel based separations, drug-protein interactions and the relative affinity of such interactions, are described in a recent review article.
has abstract
Intensity-fading MALDI is a te ...... ed in a recent review article.
@en
Link from a Wikipage to an external page
Wikipage page ID
51,272,361
Wikipage revision ID
740,765,825
comment
Intensity-fading MALDI is a te ...... ed in a recent review article.
@en
label
Intensity fading MALDI mass spectrometry
@en