RoGFP
The reduction-oxidation sensitive green fluorescent protein (roGFP) is a redox sensitive biosensor. Two cysteines were introduced into the beta barrel structure of the GFP. The oxidation state (reduced dithiol or the oxidized disulfide) of the engineered thiols determines the fluorescence properties of the sensor. Originally, different roGFP versions were presented to allow the in vivo imaging of reducing compartments such as the cytosol (roGFP2). The cysteines introduced at the amino acid positions 147 and 204 produced the most robust results. roGFP2 preferentially interacts with glutaredoxins and therefore reports the cellular glutathione redox potential. The specificity of roGFP2 for glutathione is further increased by linking it to the human glutaredoxin 1 (Grx1).By expressing the Grx1
primaryTopic
RoGFP
The reduction-oxidation sensitive green fluorescent protein (roGFP) is a redox sensitive biosensor. Two cysteines were introduced into the beta barrel structure of the GFP. The oxidation state (reduced dithiol or the oxidized disulfide) of the engineered thiols determines the fluorescence properties of the sensor. Originally, different roGFP versions were presented to allow the in vivo imaging of reducing compartments such as the cytosol (roGFP2). The cysteines introduced at the amino acid positions 147 and 204 produced the most robust results. roGFP2 preferentially interacts with glutaredoxins and therefore reports the cellular glutathione redox potential. The specificity of roGFP2 for glutathione is further increased by linking it to the human glutaredoxin 1 (Grx1).By expressing the Grx1
has abstract
The reduction-oxidation sensit ...... duction capacity of chemicals.
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roGFP (englisch Redox-sensitiv ...... reszenz redox-abhängig ändert.
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Wikipage page ID
22,064,427
Wikipage revision ID
712,050,554
hypernym
comment
The reduction-oxidation sensit ...... (Grx1).By expressing the Grx1
@en
roGFP (englisch Redox-sensitiv ...... reszenz redox-abhängig ändert.
@de
label
RoGFP
@de
RoGFP
@en