Split TEV
The split TEV technique is a molecular method to monitor protein-protein interactions in living cells. It is based on the functional reconstitution of two previously inactive fragments derived from the NIa protease of the tobacco etch virus (TEV protease). These fragments, either an N-terminal (NTEV) or C-terminal part (CTEV), are fused to protein interaction partners of choice. Upon interaction of the two candidate proteins, the NTEV and CTEV fragments get into close proximity, regain proteolytic activity, and activate specific TEV reporters which indicate an occurred protein-protein interaction.
primaryTopic
Split TEV
The split TEV technique is a molecular method to monitor protein-protein interactions in living cells. It is based on the functional reconstitution of two previously inactive fragments derived from the NIa protease of the tobacco etch virus (TEV protease). These fragments, either an N-terminal (NTEV) or C-terminal part (CTEV), are fused to protein interaction partners of choice. Upon interaction of the two candidate proteins, the NTEV and CTEV fragments get into close proximity, regain proteolytic activity, and activate specific TEV reporters which indicate an occurred protein-protein interaction.
has abstract
Das Split-TEV (‚Gespaltenes TE ...... eraktionen in lebenden Zellen.
@de
The split TEV technique is a m ...... d protein-protein interaction.
@en
Wikipage page ID
33,998,505
Wikipage revision ID
640,809,080
hypernym
type
comment
Das Split-TEV (‚Gespaltenes TE ...... eraktionen in lebenden Zellen.
@de
The split TEV technique is a m ...... d protein-protein interaction.
@en
label
Split TEV
@en
Split-TEV
@de