Tandem affinity purification
In the original version of the technique, the protein of interest with the TAP tag first binds to beads coated with IgG, the TAP tag is then broken apart by an enzyme, and finally a different part of the TAP tag binds reversibly to beads of a different type. After the protein of interest has been washed through two affinity columns, it can be examined for binding partners. Many other tag combinations have been proposed since the TAP principle was first published.
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Tandem affinity purification
In the original version of the technique, the protein of interest with the TAP tag first binds to beads coated with IgG, the TAP tag is then broken apart by an enzyme, and finally a different part of the TAP tag binds reversibly to beads of a different type. After the protein of interest has been washed through two affinity columns, it can be examined for binding partners. Many other tag combinations have been proposed since the TAP principle was first published.
has abstract
Die Tandem Affinity Purificati ...... zur stationären Phase beruhen.
@de
In the original version of the ...... principle was first published.
@en
La méthode TAP ((en)TAP-TAG) e ...... étition de la seconde colonne.
@fr
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719,046,929
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comment
Die Tandem Affinity Purificati ...... zur stationären Phase beruhen.
@de
In the original version of the ...... principle was first published.
@en
La méthode TAP ((en)TAP-TAG) e ...... étition de la seconde colonne.
@fr
label
Méthode TAP
@fr
Tandem Affinity Purification
@de
Tandem affinity purification
@en
