Methotrexate-resistant Chinese hamster ovary cells have amplified a 135-kilobase-pair region that includes the dihydrofolate reductase gene.
about
P1343
Condensation by DNA looping facilitates transfer of large DNA molecules into mammalian cellsAssignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5Purification of RIP60 and RIP100, mammalian proteins with origin-specific DNA-binding and ATP-dependent DNA helicase activitiesRecruitment of the putative transcription-repair coupling factor CSB/ERCC6 to RNA polymerase II elongation complexesA potential role for mini-chromosome maintenance (MCM) proteins in initiation at the dihydrofolate reductase replication origin.Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine.A winding road to origin discoverySpecific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.Activation of a mammalian origin of replication by chromosomal rearrangement.Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain.Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.Similar 150-kilobase DNA sequences are amplified in independently derived methotrexate-resistant Chinese hamster cells.Amplification of a cloned Chinese hamster dihydrofolate reductase gene after transfer into a dihydrofolate reductase-deficient cell lineOrganization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescueAn amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragmentsRadiation effects on DNA synthesis in a defined chromosomal replicon.Mapping of replication initiation sites in mammalian genomes by two-dimensional gel analysis: stabilization and enrichment of replication intermediates by isolation on the nuclear matrix.Multiple origins of replication in the dihydrofolate reductase amplicons of a methotrexate-resistant chinese hamster cell line.Identification and characterization of a gene that is coamplified with dihydrofolate reductase in a methotrexate-resistant CHO cell lineReplication in the amplified dihydrofolate reductase domain in CHO cells may initiate at two distinct sites, one of which is a repetitive sequence element.High-resolution mapping of replication fork movement through the amplified dihydrofolate reductase domain in CHO cells by in-gel renaturation analysis.Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells.The dihydrofolate reductase amplicons in different methotrexate-resistant Chinese hamster cell lines share at least a 273-kilobase core sequence, but the amplicons in some cell lines are much larger and are remarkably uniform in structure.Organization and genesis of dihydrofolate reductase amplicons in the genome of a methotrexate-resistant Chinese hamster ovary cell line.Mimosine, a novel inhibitor of DNA replication, binds to a 50 kDa protein in Chinese hamster cellsA general protocol for evaluating the specific effects of DNA replication inhibitors.Replication forks are associated with the nuclear matrixCharacterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.Composite patterns in neutral/neutral two-dimensional gels demonstrate inefficient replication origin usage.The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sitesLagging-strand, early-labelling, and two-dimensional gel assays suggest multiple potential initiation sites in the Chinese hamster dihydrofolate reductase originAttachment to the nuclear matrix mediates specific alterations in chromatin structure.Amplification and cloning of the Chinese hamster glutamine synthetase gene.Suicidal nucleotide sequences for DNA polymerization.Mammalian nuclei become licensed for DNA replication during late telophaseProduction of functional soluble Dectin-1 glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vectorMapping of DNA replication origins to noncoding genes of the X-inactivation center.Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zoneEvidence for rearrangement, amplification, and expression of c-myc in a human glioblastomaAmplification units containing human N-myc and c-myc genes
P2860
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P2860
Methotrexate-resistant Chinese hamster ovary cells have amplified a 135-kilobase-pair region that includes the dihydrofolate reductase gene.
description
1981 nî lūn-bûn
@nan
1981 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած
@hyw
1981 թվականի հոտեմբերին հրատարակված գիտական հոդված
@hy
1981年の論文
@ja
1981年論文
@yue
1981年論文
@zh-hant
1981年論文
@zh-hk
1981年論文
@zh-mo
1981年論文
@zh-tw
1981年论文
@wuu
name
Methotrexate-resistant Chinese ...... dihydrofolate reductase gene.
@ast
Methotrexate-resistant Chinese ...... dihydrofolate reductase gene.
@en
type
label
Methotrexate-resistant Chinese ...... dihydrofolate reductase gene.
@ast
Methotrexate-resistant Chinese ...... dihydrofolate reductase gene.
@en
prefLabel
Methotrexate-resistant Chinese ...... dihydrofolate reductase gene.
@ast
Methotrexate-resistant Chinese ...... dihydrofolate reductase gene.
@en
P2093
P2860
P356
P1476
Methotrexate-resistant Chinese ...... e dihydrofolate reductase gene
@en
P2093
J L Hamlin
N H Heintz
S M Rothman
P2860
P304
P356
10.1073/PNAS.78.10.6043
P407
P4510
P577
1981-10-01T00:00:00Z