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Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins

Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins

http://www.wikidata.org/entity/Q27641115

about

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis Recent advances in live cell imaging of hepatoma cells Crystal Structure and Raman Studies of dsFP483, a Cyan Fluorescent Protein from Discosoma striata Importance of single molecular determinants in the fidelity of expanded genetic codes Structure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206K The 1.6 Å resolution structure of a FRET-optimized Cerulean fluorescent protein Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence Directed manipulation of a flavoprotein photocycle Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study.Imaging molecular interactions in living cells.Quantitative imaging of protein interactions in the cell nucleus.Manipulation of enzyme properties by noncanonical amino acid incorporation.Stereoselective incorporation of an unsaturated isoleucine analogue into a protein expressed in E. coli.ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148.Fluorescent analogs of biomolecular building blocks: design, properties, and applications.Decoding the genome: a modified view.The photophysics of green fluorescent protein: influence of the key amino acids at positions 65, 203, and 222 The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.Rapid global fitting of large fluorescence lifetime imaging microscopy datasets.Construction of a linker library with widely controllable flexibility for fusion protein design.Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.Designing novel spectral classes of proteins with a tryptophan-expanded genetic code.Probing the active site tryptophan of Staphylococcus aureus thioredoxin with an analog.Replacement of all arginine residues with canavanine in MazF-bs mRNA interferase changes its specificity Azatryptophans endow proteins with intrinsic blue fluorescence Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo Single-fluorophore biosensors based on conformation-sensitive GFP variants.The structure and function of fluorescent proteins.Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications.Newly engineered cyan fluorescent proteins with enhanced performances for live cell FRET imaging.Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology.Prediction of two-photon absorption enhancement in red fluorescent protein chromophores made from non-canonical amino acids.The cholesterol-binding motif of the HIV-1 glycoprotein gp41 regulates lateral sorting and oligomerization.Designing logical codon reassignment - Expanding the chemistry in biology.Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging.Organic fluorine as a polypeptide building element: in vivo expression of fluorinated peptides, proteins and proteomes.Monitoring protein interactions in the living cell through the fluorescence decays of the cyan fluorescent protein.

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P2860

Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins

http://www.wikidata.org/entity/Q27641115

description

2003 nî lūn-bûn

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2003 թուականի Մայիսին հրատարակուած գիտական յօդուած

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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Expansion of the genetic code ...... of green fluorescent proteins

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S0022-2836(03)00364-4

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Journal of Molecular Biology

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Expansion of the genetic code ...... of green fluorescent proteins

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Andreas Zumbusch

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Georg Wiegand

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Gregor Jung

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Jae Hyun Bae

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Jeong Sun Kim

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M Kamran Azim

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Markus H J Seifert

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Robert Huber

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Tad A Holak

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scholarly article

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10.1016/S0022-2836(03)00364-4

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Nediljko Budisa

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10775024

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