Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants
about
Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10AX-ray crystallographic study of xylopentaose binding to Pseudomonas fluorescens xylanase ACatalysis by hen egg-white lysozyme proceeds via a covalent intermediateSubstrate specificity and subsite mobility in T. aurantiacus xylanase 10ACrystal structure and snapshots along the reaction pathway of a family 51 -L-arabinofuranosidaseThe donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolutionThe mechanisms by which family 10 glycoside hydrolases bind decorated substratesStructural and biochemical analysis of Cellvibrio japonicus xylanase 10C: how variation in substrate-binding cleft influences the catalytic profile of family GH-10 xylanasesCrystal structures of decorated xylooligosaccharides bound to a family 10 xylanase from Streptomyces olivaceoviridis E-86Functional analysis of hyperthermophilic endocellulase from Pyrococcus horikoshii by crystallographic snapshotsX-ray crystallographic studies of family 11 xylanase Michaelis and product complexes: implications for the catalytic mechanismEpoxyalkyl glycosides of D-xylose and xylo-oligosaccharides are active-site markers of xylanases from glycoside hydrolase family 11, not from family 10Long-lived glycosyl-enzyme intermediate mimic produced by formate re-activation of a mutant endoglucanase lacking its catalytic nucleophileDirected evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire.A C-terminal proline-rich sequence simultaneously broadens the optimal temperature and pH ranges and improves the catalytic efficiency of glycosyl hydrolase family 10 ruminal xylanases.Molecular modeling and MM-PBSA free energy analysis of endo-1,4-β-xylanase from Ruminococcus albus 8Direct determination of protonation states and visualization of hydrogen bonding in a glycoside hydrolase with neutron crystallographyHow similar are enzyme active site geometries derived from quantum mechanical theozymes to crystal structures of enzyme-inhibitor complexes? Implications for enzyme design.Structural determinants of the substrate specificities of xylanases from different glycoside hydrolase families.Glycosynthesis in a waterworld: new insight into the molecular basis of transglycosylation in retaining glycoside hydrolases.Synthesis and testing of mechanism-based protein-profiling probes for retaining endo-glycosidases.Role of non-covalent enzyme-substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda.Mechanism-based labeling defines the free energy change for formation of the covalent glycosyl-enzyme intermediate in a xyloglucan endo-transglycosylase.Detailed kinetic analysis and identification of the nucleophile in alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase.Structural determinants of substrate specificity in family 1 beta-glucosidases: novel insights from the crystal structure of sorghum dhurrinase-1, a plant beta-glucosidase with strict specificity, in complex with its natural substrate.Structure and function of a family 10 beta-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi Cex.An elaboration on the syn-anti proton donor concept of glycoside hydrolases: electrostatic stabilisation of the transition state as a general strategy.Homology model of a novel xylanase: molecular basis for high-thermostability and alkaline stability
P2860
Q27626007-4A891638-4824-4697-A62E-6A30E3904338Q27627412-C8DFB6D8-F3B2-4D33-AD24-15FAA2CB174FQ27634351-0135A8F3-8567-4519-AEFA-D644C6703358Q27636747-920A753E-97B2-44F8-B4AA-644D1B6CB7AEQ27642249-92DE9DEB-003C-4CC7-A214-8DB65B8A5F7CQ27642391-C5F5C3CE-9020-4C81-B7B6-1F3A6610F956Q27642761-37059480-E84E-461B-8D4B-A88721B815F8Q27642766-8D1941C5-917D-4D48-A5FA-F494F5725291Q27642768-CD162AA6-0058-4BAA-AC9E-A5C529A087E3Q27667782-0BC62C29-0369-4CAE-86AE-578E58AF57A3Q27688881-D178B32D-F396-485A-99B1-6C9DE3376971Q28344133-F95358F6-63FB-4FB2-B3FC-CEF26BF95434Q28360611-C28EDC6C-195E-43C7-BA17-5175D7825CC4Q33204723-53A8065F-B52E-4059-A7E5-2CA5DF0A7E3CQ33601727-9D69D94E-9FF1-48E3-9BE6-DF57FAF52022Q34486497-569C768C-B03E-4CD4-BB25-9F58A9851639Q36155089-A110155D-C6B6-4DB6-8CD3-9EB8DE46F65BQ36393515-5BB56EAD-51E6-48A3-8367-9CA83B5A831FQ37708271-FE1667D9-7939-466F-834B-840AF2F41259Q38385154-34EC6EA7-93BA-4710-BB16-3D9D98561EC8Q40359840-F796BAC4-B612-43B9-883C-9C57D604E998Q42997825-CA438F5D-EFD4-467D-9B15-19F14AD4FFC7Q43599092-193A541A-6A50-40AB-9CF1-63592A7B968CQ44131373-FDF8998F-2534-45E8-BEC5-99F5364391BDQ44899756-9E6F9168-9704-4ACE-B9D8-0FCA02E96325Q47780449-6AF2C4F8-B6FC-4C2C-B7FE-778C39FDD478Q49124790-AB393130-353E-4FD6-91A8-01041201599EQ57135421-6BAEE62F-1153-4046-A80D-6B935572387F
P2860
Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants
description
1998 nî lūn-bûn
@nan
1998 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
1998 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
1998年の論文
@ja
1998年論文
@yue
1998年論文
@zh-hant
1998年論文
@zh-hk
1998年論文
@zh-mo
1998年論文
@zh-tw
1998年论文
@wuu
name
Insights into transition state ...... ulation in active site mutants
@ast
Insights into transition state ...... ulation in active site mutants
@en
Insights into transition state ...... ulation in active site mutants
@nl
type
label
Insights into transition state ...... ulation in active site mutants
@ast
Insights into transition state ...... ulation in active site mutants
@en
Insights into transition state ...... ulation in active site mutants
@nl
prefLabel
Insights into transition state ...... ulation in active site mutants
@ast
Insights into transition state ...... ulation in active site mutants
@en
Insights into transition state ...... ulation in active site mutants
@nl
P2093
P2860
P356
P1476
Insights into transition state ...... ulation in active site mutants
@en
P2093
P2860
P356
10.1038/1852
P577
1998-09-01T00:00:00Z