Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
about
DNA sequence analysis with a modified bacteriophage T7 DNA polymeraseOrientation-dependent transcriptional activator upstream of a human U2 snRNA geneDirect sequencing of enzymatically amplified human genomic DNAThe thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase ISite-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase deltaStructural origins of the exonuclease resistance of a zwitterionic RNAStructural Changes in the Hydrophobic Hinge Region Adversely Affect the Activity and Fidelity of the I260Q Mutator DNA Polymerase βUnfavorable Electrostatic and Steric Interactions in DNA Polymerase β E295K Mutant Interfere with the Enzyme’s PathwayThe gene for a major exopolyphosphatase of Saccharomyces cerevisiae.DNA polymerase fidelity: comparing direct competition of right and wrong dNTP substrates with steady state and pre-steady state kineticsRPA subunit arrangement near the 3'-end of the primer is modulated by the length of the template strand and cooperative protein interactionsStructure-specific DNA-induced conformational changes in Taq polymerase revealed by small angle neutron scattering.Mutant profiles of selectable genetic elements.Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.Human U2 small nuclear RNA genes contain an upstream enhancerGenetic evidence for two protein domains and a potential new activity in bacteriophage T4 DNA polymeraseTemperature dependence and thermodynamics of Klenow polymerase binding to primed-template DNA.The thermodynamics of template-directed DNA synthesis: base insertion and extension enthalpies.Triplex formation at physiological pH: comparative studies on DNA triplexes containing 5-Me-dC tethered at N4 with spermine and tetraethyleneoxyamineInvolvement of Escherichia coli DNA Replication Proteins in Phage Lambda Red-Mediated Homologous RecombinationRe-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategiesMechanism of the idling-turnover reaction of the large (Klenow) fragment of Escherichia coli DNA polymerase I.The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD.Salt induced transitions between multiple conformations of poly (rG-m5dC).poly (rG-m5dC)Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.Inhibition of herpes simplex virus type 1 DNA polymerase activity by peptides from the UL42 accessory protein is largely nonspecificThe highly conserved U small nuclear RNA 3'-end formation signal is quite tolerant to mutation.Long-lived intracellular single-molecule fluorescence using electroporated molecules.Mechanism of DNA polymerase I: exonuclease/polymerase activity switch and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions.The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site.Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.A cII-dependent promoter is located within the Q gene of bacteriophage lambda.Subunits of human replication protein A are crosslinked by photoreactive primers synthesized by DNA polymerases.Presence of 18-A long hydrogen bond track in the active site of Escherichia coli DNA polymerase I (Klenow fragment). Its requirement in the stabilization of enzyme-template-primer complex.Simple, efficient protocol for enzymatic synthesis of uniformly 13C, 15N-labeled DNA for heteronuclear NMR studies.Highly repressible expression system for cloning genes that specify potentially toxic proteins.Complete sequence of pSC101.Experience in shotgun sequencing a 134 kilobase pair DNA molecule.
P2860
Q24605322-7751BFF3-37E7-4535-99F9-0D0A6BD7EFC7Q24616451-3EE40949-AF7F-4275-A609-17BAF46222B2Q24655900-F2616CE9-73AE-4211-B713-4B9121D8E084Q24675085-EF2ACB24-F3F7-4B47-940E-CF678938537DQ24804147-4D544BD9-5A22-4D10-9887-20AEB4A8E3E7Q27620605-F09CDF99-7573-4C64-812A-B3F35B60597DQ27678030-3C8A7F4B-EABC-409A-A3B1-5BEF90B03BA2Q27679382-0BE37A4D-DC3A-48B9-A717-4AFC12257C0CQ27931670-60362ACF-61BE-4BFE-A8E0-C660CEBC4E68Q30492695-430ED40F-F251-40D5-B39B-53F328EC91FBQ30789768-6722E14B-0F91-436A-BB18-099A06864394Q33340195-1D021018-97A2-4EC0-A536-B4F4618F2DADQ33451348-FEDA3165-A071-400E-98F6-EAAD8A9F391EQ33866298-A384C67A-FEEC-4C95-801A-6ADFCBBF3723Q33879797-2E0880B6-E347-4E82-AB2C-8CBE7C11377BQ33956131-F89A4A4E-551C-44E9-9646-5DA61C45B12EQ34354293-88CCE071-0FEE-4DAE-894D-DA8059252BFBQ34384012-66DF75EC-D84F-43E4-92D4-818BFC904E2CQ34642362-0A46112B-5225-4DE8-A354-0C4943BF310DQ34807371-B495A2E8-5C0F-4C37-A7CB-A0FC12C7E752Q35018951-BC6D0FF3-E4B4-4383-B85F-CFE7B9887D26Q35585279-E79767A2-91D0-4846-B891-5BD77B9EBD59Q35919753-0CDF8116-1007-42D5-B76A-6EC97B35BC1BQ35943171-82826941-814B-4B3F-A4EF-320E10227243Q36114389-131A3524-5363-47FC-BA0C-16DAD39CF158Q36143722-9060E6C1-9343-486B-BF79-7EA38C170F10Q36300382-9F442EF9-6CBA-4E76-B822-0064EF503152Q36639336-C4D79670-81EA-44B1-B050-63A286037B87Q36841698-6A8CB987-EEA3-4470-A7BE-AB113423CBC9Q37368213-6A4B7E84-5140-4FF5-9A6C-A0623B293A4CQ37393711-2665001B-E462-4D2E-8FF6-7E4073E2EDC2Q37536215-8BFBAB4E-FECD-4721-BCCF-6B104E31E92DQ37672339-540B44AC-DC5A-4785-9D67-1D915D990531Q37687666-14317325-3A23-4647-BB30-5025C7260648Q38339828-5347A2EA-6C93-472F-A14A-4E57A85BF3BDQ38358840-7DB997BA-6A0D-4CE6-9649-62004E2E0E35Q39724025-3A35C7EA-2EBE-4AF8-B669-25333E4BC498Q39962490-FA17E240-5DF3-48BC-9DA0-E4ACAF8A8362Q40463399-5A669135-BA6B-4F55-A629-617420AED85DQ41705880-11CFC958-7584-4F72-A8B7-CA688A926690
P2860
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
description
1983 nî lūn-bûn
@nan
1983 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
1983 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
1983年の論文
@ja
1983年論文
@yue
1983年論文
@zh-hant
1983年論文
@zh-hk
1983年論文
@zh-mo
1983年論文
@zh-tw
1983年论文
@wuu
name
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@ast
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@en
type
label
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@ast
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@en
prefLabel
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@ast
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@en
P2860
P356
P1476
Construction of a plasmid that ...... ymerase I of Escherichia coli.
@en
P2093
N D Grindley
P2860
P304
P356
10.1073/PNAS.80.7.1830
P407
P577
1983-04-01T00:00:00Z