High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

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http://www.wikidata.org/entity/Q30939329

Q30939329

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Structure-guided recombination creates an artificial family of cytochromes P450.Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59 Directed evolution: tailoring biocatalysts for industrial applications Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display.Efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of Hansenula polymorpha Diversifying carotenoid biosynthetic pathways by directed evolution.Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution.Laboratory-directed protein evolution Directed evolution: an approach to engineer enzymes.Revealing biases inherent in recombination protocols.Construction and flow cytometric screening of targeted enzyme libraries.Mutations from a family-shuffling-library reveal amino acid residues responsible for the thermostability of endoglucanase CelA from Clostridium thermocellum.PCRless library mutagenesis via oligonucleotide recombination in yeast Reiterative Recombination for the in vivo assembly of libraries of multigene pathways.Development of chimeric laccases by directed evolution.Directed evolution reveals requisite sequence elements in the functional expression of P450 2F1 in Escherichia coli Directed evolution of fungal laccases Library generation by gene shuffling.Improving industrial yeast strains: exploiting natural and artificial diversity.Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes.Exploring the potential of xenobiotic-metabolising enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes.Directed evolution of cytochrome P450 enzymes for biocatalysis: exploiting the catalytic versatility of enzymes with relaxed substrate specificity.High-throughput functional screening of steroid substrates with wild-type and chimeric P450 enzymes.Enzyme engineering toolbox – a ‘catalyst’ for change

P2860

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P2860

High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

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http://www.wikidata.org/entity/Q30939329

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2000 nî lūn-bûn

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2000 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած

@hyw

2000 թվականի հոտեմբերին հրատարակված գիտական հոդված

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2000年の論文

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2000年論文

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2000年論文

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2000年論文

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2000年論文

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2000年論文

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2000年论文

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