Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling.
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Laboratory-Evolved Enzymes Provide Snapshots of the Development of Enantioconvergence in Enzyme-Catalyzed Epoxide HydrolysisDirected Evolution and Resolution Mechanism of 1, 3-Propanediol Oxidoreductase from Klebsiella pneumoniae toward Higher Activity by Error-Prone PCR and BioinformaticsStrategies for discovery and improvement of enzyme function: state of the art and opportunitiesEngineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.Protein engineering of epoxide hydrolase from Agrobacterium radiobacter AD1 for enhanced activity and enantioselective production of (R)-1-phenylethane-1,2-diol.Rapid methods for high-throughput detection of sulfoxides.Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase.Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates.Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection methodProtein engineering of α/β-hydrolase fold enzymes.Klebsiella spp as a 1, 3-propanediol producer: the metabolic engineering approach.An Overview on the Enhancement of Enantioselectivity and Stability of Microbial Epoxide Hydrolases.Diversity and biocatalytic potential of epoxide hydrolases identified by genome analysis.Variants of the Sir4 Coiled-Coil Domain Improve Binding to Sir3 for Heterochromatin Formation in Saccharomyces cerevisiae.Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli.From molecular engineering to process engineering: development of high-throughput screening methods in enzyme directed evolution.Enhanced catalytic efficiency and enantioselectivity of epoxide hydrolase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis for (R)-epichlorohydrin synthesis.Enantioselective hydrolysis of racemic styrene oxide and its substituted derivatives using newly-isolated Sphingopyxis sp. exhibiting a novel epoxide hydrolase activity.Active site analysis of cis-epoxysuccinate hydrolase from Nocardia tartaricans using homology modeling and site-directed mutagenesis.Enhancement of epoxide hydrolase production by 60 Co gamma and UV irradiation mutagenesis of Aspergillus niger ZJB-09103.Improvement of the production efficiency of L-(+)-tartaric acid by heterogeneous whole-cell bioconversion.
P2860
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P2860
Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling.
description
2004 nî lūn-bûn
@nan
2004 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
2004 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
name
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@ast
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@en
type
label
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@ast
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@en
prefLabel
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@ast
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@en
P2093
P1476
Directed evolution of epoxide ...... r-prone PCR and DNA shuffling.
@en
P2093
Dick B Janssen
Jaap Kingma
Jeffrey H Lutje Spelberg
Marcel G Wubbolts
Theo Sonke
P304
P356
10.1016/J.CHEMBIOL.2004.04.019
P50
P577
2004-07-01T00:00:00Z