High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.
about
Microfluidic systems for biosensingSelection of restriction endonucleases using artificial cellsMicrodroplets in microfluidics: an evolving platform for discoveries in chemistry and biology.Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenasesInterplay of metagenomics and in vitro compartmentalization.A flow cytometry-based screening system for directed evolution of proteases.A high-throughput screen for antibiotic drug discoveryMicrofluidic compartmentalized directed evolution.Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions.Directed evolution of the substrate specificity of biotin ligase.One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution.An improved single cell ultrahigh throughput screening method based on in vitro compartmentalization.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Antigen-expressing immunostimulatory liposomes as a genetically programmable synthetic vaccineLiposome display for in vitro selection and evolution of membrane proteins.An efficient high-throughput screening assay for rapid directed evolution of halohydrin dehalogenase for preparation of β-substituted alcohols.An in vitro compartmentalization-based method for the selection of bond-forming enzymes from large librariesNano-enabled synthetic biology.In vitro flow cytometry-based screening platform for cellulase engineering.Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS).Selection strategies for improved biocatalysts.Directed evolution of streptavidin variants using in vitro compartmentalization.Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform.Effects of antigen-expressing immunostimulatory liposomes on chemotaxis and maturation of dendritic cells in vitro and in human skin explants.Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics.Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes.Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.High Throughput Screening and Selection Methods for Directed Enzyme EvolutionOptimization and quantification of protein synthesis inside liposomes.Bacterial cell-free expression technology to in vitro systems engineering and optimization.Throughput enhancement of parallel step emulsifier devices by shear-free and efficient nozzle clearance.Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.Activity-Fed Translation (AFT) Assay: A New High-Throughput Screening Strategy for Enzymes in Droplets.A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets.Fluorescence-activated droplet sorting of lipolytic microorganisms using a compact optical system.Ultra-high density protein spots achieved by on chip digitalized protein synthesis.Overview: methods and applications for droplet compartmentalization of biology.A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution.Microfluidic Droplet Consistency Monitoring and Cell Detection via Laser Excitation.Directed evolution of hydrolases for prevention of G-type nerve agent intoxication
P2860
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P2860
High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.
description
2005 nî lūn-bûn
@nan
2005 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2005 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
name
High-throughput screening of e ...... d sorting of double emulsions.
@ast
High-throughput screening of e ...... d sorting of double emulsions.
@en
type
label
High-throughput screening of e ...... d sorting of double emulsions.
@ast
High-throughput screening of e ...... d sorting of double emulsions.
@en
prefLabel
High-throughput screening of e ...... d sorting of double emulsions.
@ast
High-throughput screening of e ...... d sorting of double emulsions.
@en
P2093
P1476
High-throughput screening of e ...... ed sorting of double emulsions
@en
P2093
Andrew D Griffiths
Bernard T Kelly
Enrico Mastrobattista
Estelle Chanudet
Patrick Treacy
P304
P356
10.1016/J.CHEMBIOL.2005.09.016
P50
P577
2005-12-01T00:00:00Z