Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
about
High-throughput microfluidic single-cell RT-qPCRNovel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCASingle-Cell Isolation and Gene Analysis: Pitfalls and PossibilitiesPopulation transcriptomics with single-cell resolution: a new field made possible by microfluidics: a technology for high throughput transcript counting and data-driven definition of cell typesDiscrete distributional differential expression (D3E)--a tool for gene expression analysis of single-cell RNA-seq data.Massively parallel detection of gene expression in single cells using subnanolitre wells.Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol.Correction of RT-qPCR data for genomic DNA-derived signals with ValidPrime.Transcriptional activation and cell cycle block are the keys for 5-fluorouracil induced up-regulation of human thymidylate synthase expression.Nonlinear signalling networks and cell-to-cell variability transform external signals into broadly distributed or bimodal responses.Self-digitization microfluidic chip for absolute quantification of mRNA in single cells.Heterogeneity of astrocytes: from development to injury - single cell gene expressionTurbo FISH: a method for rapid single molecule RNA FISH.Single-cell analysis reveals early manifestation of cancerous phenotype in pre-malignant esophageal cells.Molecular profiling of single Sca-1+/CD34+,- cells--the putative murine lung stem cells.Quantification noise in single cell experimentsPre-amplification in the context of high-throughput qPCR gene expression experiment.Probes for intracellular RNA imaging in live cellsTranscription stochasticity of complex gene regulation models.Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq.Monitoring the single-cell stress response of the diatom Thalassiosira pseudonana by quantitative real-time reverse transcription-PCR.The workflow of single-cell expression profiling using quantitative real-time PCR.Modelling HIV-1 2-LTR dynamics following raltegravir intensification.Single-cell responses to ionizing radiation.Direct cell lysis for single-cell gene expression profilingA single molecule view of gene expression.Single-cell gene-expression profiling and its potential diagnostic applications.Mutation analysis with random DNA identifiers (MARDI) catalogs Pig-a mutations in heterogeneous pools of CD48-deficient T cells derived from DMBA-treated rats.Single-cell RNA sequencing to explore immune cell heterogeneity.Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology.Development of low cost two-step reverse transcription-quantitative polymerase chain reaction assays for rotavirus detection in foul surface water drains.The correlation between expression profiles measured in single cells and in traditional bulk samplesMethods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells.Quantitative single-cell gene expression measurements of multiple genes in response to hypoxia treatment.How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.A Double-Hybridization Approach for the Transcription- and Amplification-Free Detection of Specific mRNA on a Microarray.Analysis of gene expression at the single-cell level using microdroplet-based microfluidic technology.Modeling uncertainty in single-copy assays for HIV.Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulationsGlobal and targeted approaches to single-cell transcriptome characterization.
P2860
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P2860
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
description
2008 nî lūn-bûn
@nan
2008 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
2008 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
2008年の論文
@ja
2008年論文
@yue
2008年論文
@zh-hant
2008年論文
@zh-hk
2008年論文
@zh-mo
2008年論文
@zh-tw
2008年论文
@wuu
name
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@ast
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@en
type
label
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@ast
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@en
prefLabel
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@ast
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@en
P2860
P356
P1476
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
@en
P2093
Martin Bengtsson
Martin Hemberg
P2860
P2888
P356
10.1186/1471-2199-9-63
P577
2008-07-17T00:00:00Z
P5875
P6179
1028885990