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Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

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Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies.Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage Regeneration of Articular Cartilage by Human ESC-Derived Mesenchymal Progenitors Treated Sequentially with BMP-2 and Wnt5a.Expression microdissection adapted to commercial laser dissection instruments.SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling.Why is it crucial to reintegrate pathology into cancer research?Influence of hypoxia induced by minimally invasive prostatectomy on gene expression: implications for biomarker analysis.MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells Validation of endogenous control genes for gene expression studies on human ocular surface epithelium 2D-PCR: a method of mapping DNA in tissue sections.Identification of SOCS2 and SOCS6 as biomarkers in human colorectal cancer.Viral expression and molecular profiling in liver tissue versus microdissected hepatocytes in hepatitis B virus-associated hepatocellular carcinoma.Identification of unique expression signatures and therapeutic targets in esophageal squamous cell carcinoma.Cholangiocyte senescence by way of N-ras activation is a characteristic of primary sclerosing cholangitis Anterograde trafficking of neurotrophin-3 in the adult olfactory system in vivo.Antisense-mediated isoform switching of steroid receptor coactivator-1 in the central nucleus of the amygdala of the mouse brain.Saliva as a sampling source for the detection of leukemic fusion transcripts Scientific considerations for evaluating cancer bioassays conducted by the Ramazzini Institute Optimizing Frozen Sample Preparation for Laser Microdissection: Assessment of CryoJane Tape-Transfer System®Laser capture microdissection for the investigative pathologist.Spatial regulation of gene expression during growth of articular cartilage in juvenile mice.Identification of Site-Specific Stroke Biomarker Candidates by Laser Capture Microdissection and Labeled Reference Peptide Thyroid regeneration: characterization of clear cells after partial thyroidectomy Adaptation of Laser Microdissection Technique for the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model by NanoString Technologies.Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling.Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq.Infrared laser ablation sample transfer of tissue DNA for genomic analysis.Three-dimensional mRNA measurements reveal minimal regional heterogeneity in esophageal squamous cell carcinoma.Analysis of transcription factor mRNAs in identified oxytocin and vasopressin magnocellular neurons isolated by laser capture microdissection Measuring molecular biomarkers in epidemiologic studies: laboratory techniques and biospecimen considerations.An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing.Identification of transcription factors linked to cell cycle regulation in Arabidopsis.ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization.The increase in the expression and hypomethylation of MUC4 gene with the progression of pancreatic ductal adenocarcinoma.Loss of Myosin Vb in colorectal cancer is a strong prognostic factor for disease recurrence.Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post-mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR.Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease.Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model.

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Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

http://www.wikidata.org/entity/Q33455428

description

2009 nî lūn-bûn

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2009 թուականի Մայիսին հրատարակուած գիտական յօդուած

@hyw

2009 թվականի մայիսին հրատարակված գիտական հոդված

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2009年の論文

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2009年論文

@yue

2009年論文

@zh-hant

2009年論文

@zh-hk

2009年論文

@zh-mo

2009年論文

@zh-tw

2009年论文

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name

Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

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Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

@en

type

label

Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

@ast

Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

@en

prefLabel

Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

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Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

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Nature Protocols

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Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

@en

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Heidi S Erickson

http://www.w3.org/2001/XMLSchema#string

John W Gillespie

http://www.w3.org/2001/XMLSchema#string

Michael R Emmert-Buck

http://www.w3.org/2001/XMLSchema#string

Paul S Albert

http://www.w3.org/2001/XMLSchema#string

Peter A Pinto

http://www.w3.org/2001/XMLSchema#string

Rodrigo F Chuaqui

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W Marston Linehan

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability.

PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency.

Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

Systematic variation in gene expression patterns in human cancer cell lines

A new tumor suppressor DnaJ-like heat shock protein, HLJ1, and survival of patients with non-small-cell lung carcinoma

Altered patterns of transcription of the septin gene, SEPT9, in ovarian tumorigenesis

Laser capture microdissection

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902-922

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scholarly article

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10.1038/NPROT.2009.61

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6

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4

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Jaime Rodriguez-Canales

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2009-05-21T00:00:00Z

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26249913

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1013825533

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19478806

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2760821

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