Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.
about
Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductusRelated bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.Naturally-occurring, dually-functional fusions between restriction endonucleases and regulatory proteins.Diversity of DNA methyltransferases that recognize asymmetric target sequences.Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif.The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities.Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increaseCharacterization of cleavage intermediate and star sites of RM.Tth111II.Kinetics of Methylation by EcoP1I DNA Methyltransferase.Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic geneCharacterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI.Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site.A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.
P2860
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P2860
Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.
description
2009 nî lūn-bûn
@nan
2009 թուականի Մայիսին հրատարակուած գիտական յօդուած
@hyw
2009 թվականի մայիսին հրատարակված գիտական հոդված
@hy
2009年の論文
@ja
2009年論文
@yue
2009年論文
@zh-hant
2009年論文
@zh-hk
2009年論文
@zh-mo
2009年論文
@zh-tw
2009年论文
@wuu
name
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@ast
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@en
type
label
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@ast
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@en
prefLabel
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@ast
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@en
P2860
P356
P1476
Cloning and analysis of a bifu ...... f a Thermus sp. enzyme family.
@en
P2093
Agnieszka Zylicz-Stachula
P2860
P2888
P356
10.1186/1471-2199-10-52
P577
2009-05-29T00:00:00Z
P5875
P6179
1005750442