Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
about
To get what we aim for--progress in diversity generation methodsBiocatalyst development by directed evolutionOmniChange: the sequence independent method for simultaneous site-saturation of five codonsStabilization of a metabolic enzyme by library selection in Thermus thermophilus.A flow cytometry-based screening system for directed evolution of proteases.Engineering an Acinetobacter regulon for biosensing and high-throughput enzyme screening in E. coli via flow cytometry.A general strategy for the evolution of bond-forming enzymes using yeast displayImprovement of the fluorescence intensity during a flow cytometric analysis for rice protoplasts by localization of a green fluorescent protein into chloroplasts.Therapeutic antibody engineering by high efficiency cell screening.One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution.An improved single cell ultrahigh throughput screening method based on in vitro compartmentalization.Evolution of enzyme catalysts caged in biomimetic gel-shell beads.Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum.On-chip analysis, indexing and screening for chemical producing bacteria in a microfluidic static droplet array.Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers.Advances in Mammalian cell line development technologies for recombinant protein production.Flexibility and reactivity in promiscuous enzymes.Reporter-based screening and selection of enzymes.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Biomolecular engineering for nanobio/bionanotechnology.Actinomycetes: a repertory of green catalysts with a potential revenue resource.Engineering microbial cells for the biosynthesis of natural compounds of pharmaceutical significance.Development of a universal glycosyltransferase assay amenable to high-throughput formats.High Throughput Screening and Selection Methods for Directed Enzyme EvolutionFluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP).The application of microfluidic-based technologies in the cycle of metabolic engineering.Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets.Archimedes' principle for characterisation of recombinant whole cell biocatalysts.Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase.A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria.A combination of in vivo selection and cell sorting for the identification of enantioselective biocatalysts.Engineering a vitamin B12 high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti.Development of Biocatalytic Processes in Japan and Germany: From Research Synergies to Industrial ApplicationsDiscovery and Protein Engineering of Biocatalysts for Organic Synthesis
P2860
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P2860
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
description
2009 nî lūn-bûn
@nan
2009 թուականի Նոյեմբերին հրատարակուած գիտական յօդուած
@hyw
2009 թվականի նոյեմբերին հրատարակված գիտական հոդված
@hy
2009年の論文
@ja
2009年論文
@yue
2009年論文
@zh-hant
2009年論文
@zh-hk
2009年論文
@zh-mo
2009年論文
@zh-tw
2009年论文
@wuu
name
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@ast
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@en
type
label
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@ast
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@en
prefLabel
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@ast
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@en
P356
P1433
P1476
Ultrahigh-throughput FACS-based screening for directed enzyme evolution.
@en
P2093
Guangyu Yang
Stephen G Withers
P304
P356
10.1002/CBIC.200900384
P577
2009-11-01T00:00:00Z